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T7 Tag Peptide
本产品不向个人销售,仅用作科学研究,不用于任何人体实验及非科研性质的动物实验。
T7 Tag Peptide图片
CAS NO:245445-88-9
包装与价格:
包装价格(元)
5mg电议
25mg电议

产品介绍
T7 标签肽是一种蛋白质标签,来源于主要 T7 衣壳蛋白 gp 10 的 N 末端 11 个残基。
Cas No.245445-88-9
别名T7标签肽
化学名T7 Tag
Canonical SMILESCC(C(C(=O)NCC(=O)NCC(=O)NC(CCC(=O)N)C(=O)NC(CCC(=O)N)C(=O)NC(CCSC)C(=O)NCC(=O)O)NC(=O)C(CCSC)NC(=O)C(CO)NC(=O)C(C)NC(=O)C(CCSC)N)O
分子式C41H71N13O16S3
分子量1098.27
溶解度>54.9mg/mL in DMSO
储存条件Desiccate at -20℃
General tipsFor obtaining a higher solubility , please warm the tube at 37 ℃ and shake it in the ultrasonic bath for a while.
Shipping ConditionEvaluation sample solution : ship with blue ice
All other available size: ship with RT , or blue ice upon request
产品描述

The T7 tag is an epitope tag composed of an 11-residue peptide encoded from the leader sequence of the T7 bacteriophage gene10. This gene encodes a T7 major capsid protein whose function is not clear. Epitope tags are useful for the labeling and detection of proteins using immunoblotting, immunoprecipitation, and immunostaining techniques. Because of their small size, they are unlikely to affect the tagged protein's biochemical properties.
The T7 tag is commonly engineered onto the N- or C- terminus of a protein of interest so that the tagged protein can be analyzed and visualized using immunochemical methods. The T7 tag has been used extensively as a general epitope tag in many expression vectors including the pET system that is based on T7 RNA polymerase expression systems [1].


References:
1. Studier, F.W. and Moffatt, B.A. (1986). Use of bacteriophage T7 RNA polymerase to direct selective high-level expression of cloned genes. J Mol Biol 189, 113-30.