Hygromycin B-d₄is intended for use as an internal standard for the quantification of hygromycin B by GC- or LC-MS. Hygromycin B is an aminoglycoside antibiotic that has been found inS. hygroscopicus.^1,2It is active againstE. coli(MIC = 150 µg/ml) and cytotoxic to both prokaryotic and eukaryotic cellsviainhibition of protein synthesis.^3,4,5Hygromycin B has commonly been used in molecular and cell biology applications to select for transformed cells expressing theE. colihygromycin resistance geneshygorhph.^4,5

1.Eustice, D.C., and Wilhelm, J.M.Mechanisms of action of aminoglycoside antibiotics in eucaryotic protein synthesisAntimicrob. Agents Chemother.26(1)53-60(1984) 2.CabaÑas, M.J., VÁzquez, D., and Modolell, J.Dual interference of hygromycin B with ribosomal translocation and with aminoacyl-tRNA recognitionEur. J. Biochem.8721-27(1978) 3.McGaha, S.M., and Champney, W.S.Hygromycin B inhibition of protein synthesis and ribosome biogenesis in Escherichia coliAntimicrob. Agents Chemother.51(2)591-596(2006) 4.Kaster, K.R., Burgett, S.G., Rao, R.N., et al.Analysis of a bacterial hygromycin B resistance gene by transcriptional and translational fusions and by DNA sequencingNucleic Acids Res.11(19)6895-6911(1983) 5.Blochlinger, K., and Diggelmann, H.Hygromycin B phosphotransferase as a selectable marker for DNA transfer experiments with higher eucaryotic cellsMol. Cell Biol.4(12)2929-2931(1984)