| 包装: | 250mg |
| 市场价: | 450元 |
Cell lines | B. burgdorferi |
Preparation Method | Vancomycin was added at a final concentration of 0.5 -2.0 µg/mL into exponentially growing Borrelia spirochetes and incubated for up to 48 h. |
Reaction Conditions | 0.5-2.0 µg/mL Vancomycinfor 48 h |
Applications | Low to moderate doses of vancomycin ( 2.0 µg/mL for 24 h) produced small alterations in cell shape and that as the dose was increased, cell speed decreased. Vancomycin concentrations >1.0 µg/mL also inhibited cell growth and led to bleb formation on a fraction of the cells. |
Animal models | C57BL/6 (Th1) and BALB/c (Th2) of 6-8 weeks |
Preparation Method | Both Th1 (C57BL/6)- and Th2 (BALB/c)-biased mice were treated with vancomycin (at 50 mg per kg of body weight) for six consecutive days. 0.5 ml of vancomycin was orally gavaged twice daily at a gap of 12 h. Following the withdrawal of 6 days of treatment with vancomycin, mice were observed for 60 days. |
Dosage form | 50 mg/kg twice daily at a gap of 12 h,oral |
Applications | The gut microbiome profiling, behavior, and immune responses varied significantly between Th1- and Th2-biased mice. By withdrawing the treatment with vancomycin of major gut microbes, important physiological and behavioral changes of both mice strains returned to the normal (untreated control) level. |
| 产品描述 | Vancomycin is one of the potent antibiotics to perturb the gut microbiota[1]. Vancomycin treatment caused a significant alteration in the composition and diversity of the commensal gut microbiota of the host[2].Vancomycin specifically blocks peptide cross-linking in the bacterial peptidoglycan by recognizing terminal peptide sequences and blocking their binding site to enzymatic cross-linkers. Vancomycin has previously been shown to be active against B. burgdorferi in vitro[5,6]. Low to moderate doses of vancomycin ( 2.0 µg/mL for 24 h) produced small alterations in cell shape and that as the dose was increased, cell speed decreased. Vancomycin concentrations >1.0 µg/mL also inhibited cell growth and led to bleb formation on a fraction of the cells[3]. The addition of vancomycin at a concentration of 10ug/ml-1 to the wash solution of the cell-saver achieved similar concentrations in the autologous blood concentrate recovered allowing for bacterial removal, with negative blood cultures in all cases[4]. Metagenomic analysis of gut microbiota revealed that the treatment with vancomycin caused a significant decrease in the relative abundance of Firmicutes and Bacteroidetes phyla with a time-dependent increase in Proteobacteria and Verrucomicrobia phyla. Maximum restoration (>70%) of gut microbiota happened by the 15th day of withdrawal of vancomycin. BALB/c mice showed a more efficient restoration of gut microbiota compared to C57BL/6 mice[7]. Mice treated with vancomycin alone had a better clinical outcome in the treatment phase of CDI than mice treated with metronidazole alone[8]. References: |
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