包装 | 价格(元) |
1mL (100 rxns) | 电议 |
5mL (500 rxns) | 电议 |
25mL (2500 rxns) | 电议 |
Reagent | 10 μL |
20 μL | 50 μL |
SYBR Master mix (2×) | 5 μL |
10 μL | 25 μL |
PCR Forward Primer (10 μM) | 0.2 μL |
0.4 μL | 1 μL |
PCR Reverse Primer (10 μM) | 0.2 μL |
0.4 μL | 1 μL |
Reference Dye (optional) | 0.2 μL |
0.4 μL | 1 μL |
DNA | 1 μL |
2 μL | 4 μL |
ddH2O | 3.4 μL |
6.8 μL | 18 μL |
Total | 10 μL |
20 μL | 50 μL |
文献引用 | |
产品描述 | The Super Hot-Start SYBR Green qPCR Master Mix is a ready-to-use qPCR cocktail. It contains a novel Taq DNA Polymerase, unique hot start reagents, optimized buffer, SYBR Green I, dNTPs, PCR Enhancer and PCR stabilizer. qPCR Master Mix is provided at 2× concentration and can be used at 1× concentration by adding template, primer, passive reference dye (optional) and ddH2O. qPCR Master Mix can be directly used for robust and low-template quantitative PCR with high sensitivity, specificity and reliability. Two separate tubes of ROX Reference Dye are included for use with instruments that require a high or low level of reference dye for rectify the error of fluorescence signals between different wells. |