Cell lines

Human and rat neural progenitor cells (NPCs)

Preparation method

The solubility of this compound in DMSO is >10 mM. General tips for obtaining a higher concentration: Please warm the tube at 37℃ for 10 minutes and/or shake it in the ultrasonic bath for a while. Stock solution can be stored below -20℃ for several months.

Reaction Conditions

0, 1, 2, 5 and 10 μM; 24 or 48 hrs

Applications

(-)-Epigallocatechin Gallate (EGCG) altered human and rat NPC development in vitro. EGCG affected migration distance, migration pattern and nuclear density of NPCs growing as neurospheres. EGCG exerted these functional impairments by binding to the extracellular matrix (ECM) glycoprotein laminin, preventing its binding to β1-integrin subunits, thereby prohibiting cell adhesion and resulting in altered glia alignment and decreased number of migrating young neurons.

Animal models

A rat model of partial bladder outlet obstruction (pBOO)-induced bladder injury

Dosage form

4.5 mg/kg/day; i.p.; 2 days or 30 days

Applications

EGCG attenuated bladder inflammation caused by pBOO at the 48th hr. At the 30th day, EGCG attenuated endoplasmic reticulum (ER) stress-related apoptosis. In addition, EGCG improved bladder compliance, contractile frequency and inflammation at the 30th day.

Other notes

Please test the solubility of all compounds indoor, and the actual solubility may slightly differ with the theoretical value. This is caused by an experimental system error and it is normal.

(-)-表没食子儿茶素没食子酸酯 (EGCG) 是一种主要的儿茶素，占绿茶中儿茶素总量的 59%，是一种强大的抗氧化剂以及抗血管生成和抗肿瘤剂。EGCG 已被研究用于化学预防多种癌症，包括肝癌、胃癌、皮肤癌、肺癌、乳腺癌和结肠癌。研究结果表明，EGCG能够通过影响信号转导途径诱导细胞凋亡、促进细胞生长停滞和阻断致癌作用。此外，EGCG 对多种病毒具有抑制作用，包括 HCV、HIV-1、HBV、HSV-1、HSV-2、EBV、腺病毒、流感病毒和肠道病毒，以及多种酶，包括 DNMT、蛋白酶和 DHFR。

参考

Singh BN、Shankar S、Srivastava RK。绿茶儿茶素，表没食子儿茶素-3-没食子酸酯（EGCG）：机制、前景和临床应用。生化药理学。2011年；82（12）：1807-1821。

Steinmann J、Buer J、Pietschmann T、Steinmann E. 绿茶的一种成分表没食子儿茶素 3-没食子酸酯 (EGCG) 的抗感染特性。Br J Pharmacol。2013年；168(5):1059-1073