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Acridine Orange
本产品不向个人销售，仅用作科学研究，不用于任何人体实验及非科研性质的动物实验。
Acridine Orange图片
CAS NO:494-38-2
包装与价格：
包装价格(元)
5g电议
10g电议
25g电议

产品介绍
吖啶橙是一种可渗透细胞的荧光染料，可对生物体（细菌、寄生虫、病毒等）进行染色。
Cas No.494-38-2
别名吖啶橙,NSC 194350
化学名N3,N3,N6,N6-tetramethyl-3,6-acridinediamine
Canonical SMILESCN(C)C1=CC(N=C(C=C(N(C)C)C=C2)C2=C3)=C3C=C1
分子式C17H19N3
分子量265.4
溶解度0.3mg/mL in ethanol, 20mg/mL in DMSO, 2mg/mL in DMF
储存条件Store at -20℃, protect from light
General tipsFor obtaining a higher solubility , please warm the tube at 37 ℃ and shake it in the ultrasonic bath for a while.
Shipping ConditionEvaluation sample solution : ship with blue ice
All other available size: ship with RT , or blue ice upon request
产品描述

Acridine orange is a cell-permeable, nucleic acid-selective fluorescent cationic dye useful for cell cycle determination and detection of cellular autophagy. [1][2][3] It exhibits excitation/emission spectra of 502/525, 460/650, and 475/590 nm when bound to dsDNA, bound to ssDNA or RNA, and under acidic conditions, respectively. [1] [2] The ratio of acridine orange emission at 590 to emission at 525 nm can be used to quantify the increase in the number of acidic vesicular organelles observed during cellular autophagy.

Reference:
[1]. Virant-Klun, I., Tomazevic, T., and Meden-Vrtovec, H. Sperm single-stranded DNA, detected by acridine orange staining, reduces fertilization and quality of ICSI-derived embryos. J.Assist.Reprod.Genet. 19(7), 319-328 (2002).
[2]. Han, J., and Burgess, K. Fluorescent indicators for intracellular pH. Chem. Revs. 110(5), 2709-2728 (2010).
[3]. Thomé, M.P., Filippi-Chiela, E.C., Villodre, E.S., et al. Ratiometric analysis of Acridine Orange staining in the study of acidic organelles and autophagy. J. Cell Sci. 129(24), 4622-4632 (2016).