| 包装 | 价格(元) |
| 100mg | 电议 |
| 200mg | 电议 |
Cell lines | Epithelial ovarian cancer (EOC) cells |
Preparation method | The solubility of this compound in DMSO is >10 mM. General tips for obtaining a higher concentration: Please warm the tube at 37 ℃ for 10 minutes and/or shake it in the ultrasonic bath for a while. Stock solution can be stored below - 20 ℃ for several months. |
Reacting condition | 0 ~ 80 μM; 48 hrs |
Applications | In a series of EOC cells, Emodin inhibited cell proliferation in a dose-dependent manner. Emodin at the dose of 20 μM abrogated the migration and invasion abilities of A2780 and SK-OV-3 cells transfected with pLVX-ILK. The results of western blotting analysis implied that Emodin inhibited the migration and invasion abilities of EOC cells by blocking epithelial-mesenchymal transition (EMT) through targeting integrin-linked kinase (ILK). |
Animal models | Nude mice bearing SK-OV-3/pLVX-ILK cells |
Dosage form | 50 mg/kg/d; i.p.; for 21 days |
Applications | In nude mice bearing SK-OV-3/pLVX-ILK cells, Emodin significantly inhibited tumor growth. Meanwhile, Emodin counteracted the effects of over-expression of ILK on EOC cells. The immunohistochemical results demonstrated that Emodin inhibited the EMT of tumor cells. In addition, Emodin exhibited mild cardiac, liver and renal toxicities in vivo. |
Other notes | Please test the solubility of all compounds indoor, and the actual solubility may slightly differ with the theoretical value. This is caused by an experimental system error and it is normal. |
| 产品描述 | Description: IC50 Value: observed at 72 hr were as follows: 66.9 uM, Hep3B cells; 74.36 uM, HepG2 cells; and 101.5 uM, Huh7 cells [1]. Emodin (1,3,8-trihydroxy-6-methylanthraquinone) is a naturally occurring anthraquinone present in the roots and barks of numerous plants, molds, and lichens, and an active ingredient of various Chinese herbs. Emodin exerts antiproliferative effects in cancer cells that are regulated by different signaling pathways. in vitro: At 6h after emodin treatment, the levels of GDF15, CYP1A1, CYP1B1, and CYR61 were upregulated [1]. Emodin increased the resting tension of gallbladder smooth muscle strips and inhibited voltage-dependent K(+) current in a dose-dependent manner. When 10 microM emodin was applied to gallbladder smooth muscle cells for 3-6 min., the amplitude of voltage-dependent K(+) current was decreased by 31.5 +/- 0.5% at +40 mV, and this inhibitory effect mostly recovered after washout [2]. in vivo: Emodin treatment significantly alleviated the severity of the disease, based on the reduced hind paw swelling and clinical scores, compared with untreated CIA mice. Comparing with untreated CIA mice, emodin treatment inhibited the levels of TNF-α and IL-6 in the plasma, PGE2 production, and COX-2 protein expression in synovial tissues in a dose manner [3]. Clinical trial: N/A |
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