Broad spectrum cysteine reactive probe. Covalently binds cysteine residues in proteins. Can be utilized for covalent fragment-based ligand discovery (FBLD) and to identify Zn2+-binding cysteines. Used as a click-chemistry handle to attach a fluorophore or biotin to reactive cysteines in proteins for visualization by in-gel fluorescence or identification by mass spectrometry, respectively. Covalently binds cysteine residues in and activates TRPA1 channels.

Backus et al (2016) Proteome-wide covalent ligand discovery in native biological systems. Nature 534 570 PMID:27309814 |Pace and Weerapana et al (2014) A competitive chemical-proteomic platform to identify zinc-binding cysteines. ACS Chem.Biol. 9 258 PMID:24111988 |Macpherson et al (2007) Noxious compounds activate TRPA1 ion channels through covalent modification of cysteines. Nature 445 541 PMID:17237762