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PHTPP
本产品不向个人销售,仅用作科学研究,不用于任何人体实验及非科研性质的动物实验。
PHTPP图片
包装与价格:
包装价格(元)
10mg电议
50mg电议

产品介绍
PHTPP 是一种选择性雌激素受体 β (ERβ) 拮抗剂,其选择性是 ERα 的 36 倍。

Cell experiment:

Cells are plated at a density of 3 to 4×105 in 1 mL of medium per well in a 24-well plate for cell viability and cAMP analysis. Cells are cultured in Dulbecco’s Modified Eagle Medium with PHTPP (1 μM), or ethanol (0.1%) as the vehicle. The incubator is set to an atmosphere of 5% CO2 in air at 37℃, and cultures are allowed to acclimate for at least 24 h. The culture medium is then aspirated and replaced with serum-free DMEM/F12 containing 0.1 μM androstenedione. The cells are collected for intracellular cAMP and to test cell viability[1].

产品描述

PHTPP is a selective ERβ antagonist.

PHTPP is a selective ERβ antagonist. PHTPP reduces FSH-mediated cAMP production by 80% (pIgf1 mRNA expression, and facilitates the DPN-induced increase in class 1 Igf1 mRNA expression[2].

References:
[1]. Compton DR, et al. Pyrazolo[1,5-a]pyrimidines: estrogen receptor ligands possessing estrogen receptor beta antagonist activity. J Med Chem. 2004 Nov 18;47(24):5872-93.
[2]. Ogo Y, et al. IGF-1 gene expression is differentially regulated by estrogen receptors α and β in mouse endometrial stromal cells and ovarian granulosa cells. J Reprod Dev. 2014;60(3):216-23. Epub 2014 Mar 25.