包装 | 价格(元) |
10mg | 电议 |
50mg | 电议 |
Cell experiment: | Cells are plated at a density of 3 to 4×105 in 1 mL of medium per well in a 24-well plate for cell viability and cAMP analysis. Cells are cultured in Dulbecco’s Modified Eagle Medium with PHTPP (1 μM), or ethanol (0.1%) as the vehicle. The incubator is set to an atmosphere of 5% CO2 in air at 37℃, and cultures are allowed to acclimate for at least 24 h. The culture medium is then aspirated and replaced with serum-free DMEM/F12 containing 0.1 μM androstenedione. The cells are collected for intracellular cAMP and to test cell viability[1]. |
产品描述 | PHTPP is a selective ERβ antagonist. PHTPP is a selective ERβ antagonist. PHTPP reduces FSH-mediated cAMP production by 80% (pIgf1 mRNA expression, and facilitates the DPN-induced increase in class 1 Igf1 mRNA expression[2]. References: |