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BODIPY-Cholesterol
本产品不向个人销售,仅用作科学研究,不用于任何人体实验及非科研性质的动物实验。
BODIPY-Cholesterol图片
包装与价格:
包装价格(元)
500μg电议
1mg电议
5mg电议
10mg电议

产品介绍
BODIPY-胆固醇是一种具有荧光 BODIPY 基团的固有亲脂性和细胞渗透性胆固醇类似物。

Cell lines

Human primary fibroblasts

Preparation Method

NPC1‐deficient GM3123 fibroblasts were incubated with Rhodamine‐dextran overnight to label terminal endocytic compartments, followed by incubation with 5 μg/mL (8.7 μm) BODIPY-Cholesterol in normal growth medium containing 5% fetal calf serum for 4 h, or 0.5 μm BODIPY-Cholesterol in medium containing 5% lipoprotein‐starved serum for 23 h.

Reaction Conditions

5 μg/mL, 4h

Applications

Time‐dependent partitioning of BODIPY-Cholesterol in the lysosomes of human primary fibroblasts.

Cell lines

NIH 3T3

Preparation Method

NIH 3T3 were cultured in 24-well plates, incubated for 2 h with 2.5 μM BODIPY-cholesterol, and rinsed. Physiological buffer with or without 30 nM ShhN was added to the wells, and the BODIPY fluorescence intensity of the supernatants was measured after 1 h.

Reaction Conditions

2.5 μM; 2 h

Applications

The BODIPY fluorescence measured in the supernatants after 1 h was significantly lower in wells where ShhN protein was added compared to wells that did not contain ShhN.

文献引用
产品描述

BODIPY-Cholesterol is cholesterol tagged with a boron dipyrromethene difluoride (BODIPY) fluorophore used for monitoring sterol uptake and inter-organelle sterol flux in cells with excitation of 480 nm and emission of 508 nm.[1]The excretion of BODIPY cholesterol from late endoplasmic organelles depends on acidic lipase and Niemann pickc1 protein.[6]

In vitro, treatment with Bodipy-cholesterol in cells, prominent PM labeling is observed at 2–5 min; however, upon ≥30 min incubations, it is also observed the fluorescence labeling of intracellular structures.[2]In vitro efficacy test it suggested that accumulation of BODIPY-cholesterol in the media gave more reproducible values than assaying for the loss of the compound from the cells.[3]With 1 μg BODIPY-cholesterol analogs show a similar cellular localization in HeLa cells and exhibit similar cholesterol efflux properties from THP1 cells to HDL acceptors.[4]BODIPY-cholesterol efflux clearly increased when treatment of fibroblasts with the Hh pathway agonist SAG, which enhances Ptc protein expression, or over-expression of human Ptc in yeast.[5]Treatment with miR-758 inhibitor obviously increased ABCA1-dependent cholesterol efflux by BODIPY-Cholesterol efflux assay.[7]Micrographs of EPCs incubated with HDL labeld bodipy-cholesterol (50 μg/ml) 30 min, small cytoplasmic vesicles as well as large positive MVBs containing intraluminal microvesicles, tightly-packed with reaction products could be displayed. The internalized-HDL-derived bodipy-cholesterol was also spread within many of the stacked Golgi cisterns and the TGN.[8]

References:
[1].Wüstner D, et al. Potential of BODIPY-cholesterol for analysis of cholesterol transport and diffusion in living cells. Chem Phys Lipids. 2016 Jan;194:12-28.
[2].Holtta-Vuori M, et al. BODIPY-cholesterol: a new tool to visualize sterol trafficking in living cells and organisms. Traffic. 2008 Nov;9(11):1839-49.
[3].Sankaranarayanan S, et al. A sensitive assay for ABCA1-mediated cholesterol efflux using BODIPY-cholesterol. J Lipid Res. 2011 Dec;52(12):2332-2340.
[4].Liu Z, et al. Synthesis of cholesterol analogues bearing BODIPY fluorophores by Suzuki or Liebeskind-Srogl cross-coupling and evaluation of their potential for visualization of cholesterol pools. Chembiochem. 2014 Sep 22;15(14):2087-96.
[5].Bidet M, et al. The hedgehog receptor patched is involved in cholesterol transport. PLoS One. 2011;6(9):e23834.
[6].Kanerva K, et al. LDL cholesterol recycles to the plasma membrane via a Rab8a-Myosin5b-actin-dependent membrane transport route. Dev Cell. 2013 Nov 11;27(3):249-62.
[7].Yao Y, et al. Glucagon-like peptide-1 contributes to increases ABCA1 expression by downregulating miR-758 to regulate cholesterol homeostasis. Biochem Biophys Res Commun. 2018 Mar 4;497(2):652-658.
[8].Srisen K, et al. Human endothelial progenitor cells internalize high-density lipoprotein. PLoS One. 2013 Dec 30;8(12):e83189.