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Calcein-AM(Calcein acetoxymethyl ester)
本产品不向个人销售,仅用作科学研究,不用于任何人体实验及非科研性质的动物实验。
Calcein-AM(Calcein acetoxymethyl ester)图片
包装与价格:
包装价格(元)
500μg电议
1mg电议

产品介绍
Calcein-AM (Calcein acetoxymethyl ester) 是一种可渗透细胞的荧光染料,用于确定细胞活力。

Cell lines

EPC cells

Preparation Method

Uptake kinetics of EPC cells loaded with 5 μM calcein AM seeded at 1x105cells well-1and cultured at 15 ℃. Calcein AM uptake was measured as fluorescence intensity (FI).

Reaction Conditions

5 μM at 15 ℃, 1-8h

Applications

Uptake kinetics showed that for EPC cells seeded at a density of 1x105cells well-1calcein AM labelling increased throughout the 7 h tested.

文献引用
产品描述

Calcein-AM is a highly lipophilic vital dye that rapidly enters viable cells. It is converted by intracellular esterases to calcein that produces an intense green (530-nm) signal, and is retained by cells with intact plasma membrane.[1]

In vitro experiment it shown that calcein-AM assay used to assess human RBC viability after incubation (37℃ for 3 and 20 h) in the presence of Ca2+ (2.5 mM) and ionophore A 23187 (0.5 μM).[1]0.05 μM was the optimal concentration of CAM (Calcein-AM) for staining effector cells by testing 0.05, 0.1, 0.2, and 0.4 μM. Using 0.05 μM CAM to stain the PBMCs and expanded NK cells from three normal volunteers, the results demonstrated that there is no significant decrease in cytotoxicity and CAM staining had no significant effect on human NK cell activity in PBMCs or in expanded NK cells.[2]In vitro, 50μm calcein AM's fluorescent signal of 1 x lo5 lymphocytes was close to the saturation level, while the signal emitted by lymphocytes labeled with 20μm calcein AM was only slightly lower.[3]Calcein-AM has cytotoxic activity against human tumor cell lines (such as the human lymphoma U-937-GTB) at low concentrations (2.5 ug/ml).[4]In vitro experiment it demonstrated that in the mixed macrophages and THP-1 cells (5x105 cells/ml), Calcein-AM (2 μM)/propidium Iodide (PI) (4.5 μM) staining assay Calcein-AM/PI double staining was used to quantify the number of living and dead cells as a cell death assay.[5]In addition, The cells in OA chondrocytes were seeded in 24-well plates (2 × 104 cells/well), cultured for 4 h, the cells were treated with 5 μL Calcein-AM (2 μM) and 5 μL PI (2 μM) at 37℃ in conditions void of light for 30 min, and then analyzed under a fluorescence microscope.[6]

References:
[1].Bratosin D, et al. Novel fluorescence assay using calcein-AM for the determination of human erythrocyte viability and aging. Cytometry A. 2005 Jul;66(1):78-84.
[2].Jang YY, et al. An improved flow cytometry-based natural killer cytotoxicity assay involving calcein AM staining of effector cells. Ann Clin Lab Sci. 2012 Winter;42(1):42-9.
[3].Braut-Boucher F, et al. A non-isotopic, highly sensitive, fluorimetric, cell-cell adhesion microplate assay using calcein AM-labeled lymphocytes. J Immunol Methods. 1995 Jan 13;178(1):41-51.
[4].Liminga G, et al. Cytotoxic effect of calcein acetoxymethyl ester on human tumor cell lines: drug delivery by intracellular trapping. Anticancer Drugs. 1995 Aug;6(4):578-85.
[5].Xiang N, et al. Gardnerella vaginalis induces NLRP3 inflammasome-mediated pyroptosis in macrophages and THP-1 monocytes. Exp Ther Med. 2021 Oct;22(4):1174.
[6].Zhang L, et al. MicroRNA-140-5p represses chondrocyte pyroptosis and relieves cartilage injury in osteoarthritis by inhibiting cathepsin B/Nod-like receptor protein 3. Bioengineered. 2021 Dec;12(2):9949-9964.