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Necrostatin-1(Nec-1)
本产品不向个人销售,仅用作科学研究,不用于任何人体实验及非科研性质的动物实验。
Necrostatin-1(Nec-1)图片
CAS NO:4311-88-0
规格:≥98%
包装与价格:
包装价格(元)
10mg电议
25mg电议
50mg电议
100mg电议
250mg电议
500mg电议
1g电议

产品介绍
理化性质和储存条件
Molecular Weight (MW)259.33
FormulaC13H13N3OS
CAS No.4311-88-0
Storage-20℃ for 3 years in powder form
-80℃ for 2 years in solvent
Solubility (In vitro)DMSO: 51 mg/mL warming (196.66 mM)
Water:<1 mg/mL (slightly soluble or insoluble)
Ethanol: <1 mg/mL (slightly soluble or insoluble)
SynonymsNecrostatin-1; Necrostatin1; Necrostatin 1; Nec-1; Nec 1; Nec1
Chemical Name5-(1H-indol-3-ylmethyl)-3-methyl-2-sulfanylideneimidazolidin-4-one
实验参考方法
In VitroNecrostatin-1 (1-100 μM) inhibits the autophosphorylation of overexpressed and endogenous RIP1.It is found RIP1 is the primary cellular target responsible for the antinecroptosis activity of Necrostatin-1. Necrostatin-1 efficiently suppresses necroptotic cell death triggered by an array of stimuli in a variety of cell types.
In VivoNecrostatin-1 (Nec-1) induces tubular bilation and affects the kinetics of the dilation of peritubular capillaries after RCM application. Upon a single intraperitoneal application of a single dose of Necrostatin-1 (1.65 mg/kg body weight, i.p.) 15 minutes before RCM, the return to baseline levels is prevented within the observation period.
Animal modelMice: 8-10 week old male C57BL/6 mice (average weight approx.23 g) are used. Mice receive intravenous application of 200 μL PBS or radiocontrast media (RCM) via the tail vein. A single dose of Z-VAD-fmk (10 mg/kg body weight) or Necrostatin-1 (1.65 mg/kg body weight) is applied intraperitoneally 15 min. before RCM-injection. Mice are harvested another 24 hours after RCM-application (48 hours after reperfusion). Blood samples are obtained from retroorbital bleeding and serum levels of urea and creatinine are determined.
Formulation & Dosage1.65 mg/kg; i.p.
ReferencesDegterev A, et al. Nat Chem Biol, 2005, 1(2), 112-119.; J Am Soc Nephrol. 2013 Oct;24(10):1545-57.
生物活性