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4-Nitrophenyl-N-acetyl-β-D-glucosaminide
本产品不向个人销售，仅用作科学研究，不用于任何人体实验及非科研性质的动物实验。
4-Nitrophenyl-N-acetyl-β-D-glucosaminide图片
CAS NO:3459-18-5
包装与价格：
包装价格(元)
100mg电议
250mg电议
500mg电议
1g电议

产品介绍
A chromogenic substrate for N-acetyl-β-glucosaminidase
Cas No.3459-18-5
别名4-硝基苯基-2-乙酰胺基-2-脱氧-B-D-吡喃葡糖霉
Canonical SMILESO[C@H]([C@H]1NC(C)=O)[C@H](O)[C@@H](CO)O[C@H]1OC2=CC=C([N+]([O-])=O)C=C2
分子式C14H18N2O8
分子量342.3
溶解度DMF: 15mg/mL,DMF:PBS (pH 7.2) (1:4): 0.2mg/mL,DMSO: 3mg/mL
储存条件Store at -20°C
General tipsFor obtaining a higher solubility , please warm the tube at 37 ℃ and shake it in the ultrasonic bath for a while.
Shipping ConditionEvaluation sample solution : ship with blue ice
All other available size: ship with RT , or blue ice upon request
产品描述

4-Nitrophenyl-N-acetyl-β-D-glucosaminide is a chromogenic substrate for N-acetyl-β-glucosaminidase.1,2 Hydrolysis of 4-nitrophenyl-N-acetyl-β-D-glucosaminide by N-acetyl-β-glucosaminidase releases 4-nitrophenol, which can be quantified by colorimetric detection at 405 nm as a measure of N-acetyl-β-glucosaminidase activity. It has been used to quantify the activity of N-acetyl-β-D-glucosaminidase in human serum and urine in parenterally fed patients.3

|1. Borooah, J., Leaback, D.H., and Walker, P.G. Studies on glucosaminidase. 2. Substrates for N-acetyl-β-glucosaminidase. Biochem. J. 78(1), 106-110 (1961).|2. Bowers, G.N., Jr., McComb, R.B., Christensen, R.G., et al. High-purity 4-nitrophenol: Purification, characterization, and specifications for use as a spectrophotometric reference material. Clin. Chem. 26(6), 724-729 (1980).|3. Raczkowska, K., Zalewska-Szajda, B., Raczkowski, K., et al. The activity of N-acetyl-β-D-hexosaminidase in serum and urine of parenterally fed patients. Exp. Clin. Hepatology 9, 1-4 (2013).