Animal experiment:

Specific pathogen-free female 6 weeks old mice are used. The animals are maintained in a controlled room (temperature 23±2 ℃, humidity 55±15%, 12 h light cycle). After 1 week, the mice arer andomly divided into 3 groups, untreated group-receive vehicle (distill water) (Normal, n=5); DfE cream treated mice (100 mg/mouse) is divided into two groups and each receives vehicle (distill water) (AD, n=5) or Tannic acid (80 mg/kg/day, per oral) (AD+Tannic acid, n=5) and are allowed free access to drinking water and standard laboratory diet[2].

Tannic acid is a novel hERG channel blocker with IC50 of 3.4 μM.

During the course of treatment, tannic acid significantly ameliorates these phenotypes in AD skin lesions. Tannic acid treatment aslo reduces these dermal changes compare with AD mice. Treatment with tannic acid increases PPARγ expression in AD skin sections. The PPARγ protein expression is suppressed in vehicle-treated AD mice, but when treats with tannic acid, its expression is increased dramatically. The IL-1β, TNFα, TNFR1, and COX2 protein expressions are significantly up-regulated in vehicle-treated AD mice, but significantly suppressed by tannic acid treatment[2].

[1]. Xi Chu, et al. Effects of Tannic Acid, Green Tea and Red Wine on hERG Channels Expressed in HEK293 Cells. PLoS One. 2015; 10(12): e0143797. [2]. Karuppagounder V, et al. Tannic acid modulates NFκB signaling pathway and skin inflammation in NC/Nga mice through PPARγ expression. Cytokine. 2015 Dec;76(2):206-13.