| 包装 | 价格(元) |
| 10ul (5 mM) | 电议 |
| 200ul (5 mM) | 电议 |
| Physical Appearance | Solution |
| Storage | Store at -20°C or below |
| M.Wt | 946.7 (free acid) |
| Formula | C32H53N8O17P3S (free acid) |
| Synonyms | Biotin-16-Aminoallyl-2'-dCTP, Biotin-16-AA-dCTP |
| Chemical Name | lithium (E)-(5-(4-amino-2-oxo-5-(3-(4-(6-(5-(2-oxohexahydro-1H-thieno[3,4-d]imidazol-4-yl)pentanamido)hexanamido)butanamido)prop-1-en-1-yl)pyrimidin-1(2H)-yl)-3-hydroxytetrahydrofuran-2-yl)methyl triphosphate |
| Canonical SMILES | NC(C(/C=C/CNC(CCCNC(CCCCCNC(CCCCC1C(NC(N2)=O)C2CS1)=O)=O)=O)=CN3C4CC(O)C(COP([O-])(OP([O-])(OP([O-])([O-])=O)=O)=O)O4)=NC3=O.[Li+].[Li+].[Li+].[Li+] |
| 运输条件 | 蓝冰运输或根据您的需求运输。 |
| 一般建议 | 为了使其更好的溶解，请用37℃加热试管并在超声波水浴中震动片刻。不同厂家不同批次产品溶解度各有差异，仅做参考。若实验所需浓度过大至产品溶解极限，请添加助溶剂助溶或自行调整浓度。溶液形式一般不宜长期储存，请尽快用完。 |
Biotin-16-dCTP is a modified deoxynucleoside triphosphate (dNTP), serving as a convenient tool for the enzymatic introduction of the biotin moiety into a nucleic acid target of interest. The biotin moiety exhibits a very strong affinity for streptavidin. The strength and specificity of this interaction has been exploited in a variety of biological applications, such as secondary label introduction and affinity isolation. For the biotinylated nucleotides, biotin-16-dCTP and biotin-16-dUTP, strong amplicon formation can be observed with modified nucleotides substituted for 75% of their natural counterparts (i.e. dCTP and dUTP). Compared with biotin-16-dUTP whose yield decreases quickly above 75% substitution, the yield of biotin-16-dCTP decreases at a much higher substitution of ~90%. It turns out thatTaqDNA polymerase can incorporate biotin-16-dCTP with greater efficiency than biotin-16-dUTP.
Reference:
1. Natasha P, Joyclyn Y. PCR incorporation of modified dNTPs: the substrate properties of biotinylated dNTPs. BioTechniques, 2010, 48(4): 333 - 334.
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