| CAS NO: | 211990-57-7 |
| 包装 | 价格(元) |
| 5mg | 电议 |
| 25mg | 电议 |
| Physical Appearance | A solid |
| Storage | Store at -20°C |
| M.Wt | 729.65 |
| Cas No. | 211990-57-7 |
| Formula | C31H38F3N5O12 |
| Solubility | ≥73 mg/mL in DMSO; insoluble in H2O; ≥2.84 mg/mL in EtOH with ultrasonic |
| Chemical Name | 4-(sec-butyl)-7-(2-carboxyethyl)-10-(1-hydroxyethyl)-2,5,8,11-tetraoxo-13-((2-oxo-4-(trifluoromethyl)-2H-chromen-7-yl)carbamoyl)-3,6,9,12-tetraazapentadecan-15-oic acid |
| Canonical SMILES | O=C(O)CCC(NC(C(NC(C)=O)C(C)CC)=O)C(NC(C(O)C)C(NC(CC(O)=O)C(NC1=CC(O2)=C(C=C1)C(C(F)(F)F)=CC2=O)=O)=O)=O |
| 运输条件 | 蓝冰运输或根据您的需求运输。 |
| 一般建议 | 为了使其更好的溶解，请用37℃加热试管并在超声波水浴中震动片刻。不同厂家不同批次产品溶解度各有差异，仅做参考。若实验所需浓度过大至产品溶解极限，请添加助溶剂助溶或自行调整浓度。溶液形式一般不宜长期储存，请尽快用完。 |
Ac-IETD-AFC 是一种荧光显色的caspase-8的底物，包含氨基酸序列IETD，是caspase-8传导的蛋白水解的目标。同时也是caspase-3 processing enzyme, caspase-10 和granzyme b的底物。Caspase的活性可以由荧光检测游离的AFC来决定（Ex. = 400 nm，Em. = 505 nm）. 在凋亡的细胞裂解物中，Ac-IETD-AFC还能够用于鉴别和量化caspase 8的活性，用来研究caspase-8被激活后下游的信号通路。
References:
1. Mashima T, Naito M, Kataoka S, Kawai H, Tsuruo T. Aspartate-based inhibitor of interleukin-1 beta-converting enzyme prevents antitumor agent-induced apoptosis in human myeloid leukemia U937 cells. Biochem Biophys Res Commun. 1995; 209(3):907-915.
2. Nicholson DW, Ali A, Thornberry NA, et al. Identification and inhibition of the ICE/CED-3 protease necessary for mammalian apoptosis. Nature. 1995; 376(6535):17-18.
3. Thornberry NA, Lazebnik Y. Caspases: enemies within. Science. 1998; 281(5381):1312-1316.
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