Piperlonguminine

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Piperlonguminine

本产品不向个人销售，仅用作科学研究，不用于任何人体实验及非科研性质的动物实验。

CAS NO:

5950-12-9

包装与价格：

包装	价格(元)
10mg	电议
25mg	电议
50mg	电议

产品介绍

化学性质

Physical Appearance	A crystalline solid
Storage	Store at -20°C
M.Wt	273.3
Cas No.	5950-12-9
Formula	C₁₆H₁₉NO₃
Synonyms	N-Isobutylpiperamide\|NSC 125178
Solubility	≤3mg/ml in ethanol;20mg/ml in DMSO;20mg/ml in dimethyl formamide
Chemical Name	5-(1,3-benzodioxol-5-yl)-N-(2-methylpropyl)-2E,4E-pentadienamide
Canonical SMILES	O=C(/C=C/C=C/C1=CC=C(OCO2)C2=C1)NCC(C)C
运输条件	蓝冰运输或根据您的需求运输。
一般建议	为了使其更好的溶解，请用37℃加热试管并在超声波水浴中震动片刻。不同厂家不同批次产品溶解度各有差异，仅做参考。若实验所需浓度过大至产品溶解极限，请添加助溶剂助溶或自行调整浓度。溶液形式一般不宜长期储存，请尽快用完。

资料参考

Piperlonguminine is an agent with anticancer, antihyperlipidemic, as well as anti-inflammatory properties.

Piperlonguminine is a bio-active isolate of Piper longum.

In vitro: In a previous study, piperlonguminine was discovered to inhibit melanin production in melanoma B16 cells stimulated with α-MSH, 3-isobutyl-1-methylxanthine or protoporphyrin IX, where piperlonguminine showed stronger depigmenting efficacy. However, piperlonguminine could not alter1-oleoyl-2-acetyl-sn-glycerol-induced melanogenesis and could not affect protein kinase C-mediated melanin production. In additioin, piperlonguminine was not able to inhibit the catalytic activity of cell-free tyrosinase from melanoma B16 cells, and such effect was attributed to the inhibitory action of piperlonguminine on α-MSH-induced signaling via cAMP to the cAMP responsive element binding protein [1].

In vivo: In vivo, rats were subjected to middle cerebral artery occlusion for 1h, followed by reperfusion for 23 h. The results showed that the intraperitoneal injection of piperlonguminine PE at 2.4 mg/kg was able to produce a significant neuroprotective potential in rats with cerebral ischemia. In addition, piperlonguminine could attenuate the neurological deficit scores, brain infarct volume and brain water content, and could inhibit the activation of NF-κB and MAPK [2].

Clinical trial: Up to now, piperlonguminine is still in the preclinical development stage.

References:
[1] Kim KS, Kim JA, Eom SY, Lee SH, Min KR, Kim Y. Inhibitory effect of piperlonguminine on melanin production in melanoma B16 cell line by downregulation of tyrosinase expression. Pigment Cell Res. 2006 Feb;19(1):90-8.
[2] Yang T, Sun S, Wang T, Tong X, Bi J, Wang Y, Sun Z. Piperlonguminine is neuroprotective in experimental rat stroke. Int Immunopharmacol. 2014 Dec;23(2):447-51.

试验操作

细胞实验 [1]：
细胞系	黑色素瘤B16细胞
溶解方法	在DMSO中的溶解度≤20mg/ml 。为了获得更高的浓度，可以将离心管在37℃加热10分钟和/或在超声波浴中震荡一段时间。原液可以在-20℃以下储存几个月。
反应条件	3～30 μM
应用	Piperlonguminine不能改变1-油酰基-2-乙酰基-sn-甘油诱导的黑色素生成，也不能影响蛋白激酶C介导的黑色素的产生。此外，Piperlonguminine不能抑制黑色素瘤B16细胞无细胞酪氨酸酶的催化活性，这种作用归因于Piperlonguminine对cAMP到cAMP反应元件结合蛋白的α-MSH诱导信号的抑制作用。
动物实验 [2]：
动物模型	诱导的脑缺血大鼠
剂量	2.4 mg/kg (i.p.)
应用	腹腔注射 piperlonguminine（2.4 mg / kg）对脑缺血大鼠具有明显的神经保护作用。 Piperlonguminine减轻了大鼠的神经功能缺损指标，脑梗塞体积和脑含水量，并抑制了NF-κB和MAPK的活化。这些数据表明，piperlonguminine通过减轻血脑屏障（BBB）的破坏来保护大脑免受缺血性脑损伤的破坏，而血脑屏障的破坏可能是通过抑制NF-κB和MAPK信号通路来介导的。
注意事项	请测试所有化合物在室内的溶解度，实际溶解度和理论值可能略有不同，这是由实验系统的误差引起的，属于正常现象。
References: [1]. Kim KS, Kim JA, Eom SY, Lee SH, Min KR, Kim Y. Inhibitory effect of piperlonguminine on melanin production in melanoma B16 cell line by downregulation of tyrosinase expression. Pigment Cell Res. 2006 Feb;19(1):90-8. PubMed PMID: 16420250. [2]. Yang T, Sun S, Wang T, Tong X, Bi J, Wang Y, Sun Z. Piperlonguminine is neuroprotective in experimental rat stroke. Int Immunopharmacol. 2014 Dec;23(2):447-51. doi: 10.1016/j.intimp.2014.09.016. Epub 2014 Sep 22. PubMed PMID: 25257731.