| 包装 | 价格(元) |
| 10mM (in 1mL DMSO) | 电议 |
| 10mg | 电议 |
| 50mg | 电议 |
| 500mg | 电议 |
Cell lines | HepG2 cells |
Preparation Method | HepG2 cells were treated with different dosages of DAPT (25, 50, and 100 μM, n=3), with HepG2 cells incubated with blank medium as the control group. |
Reaction Conditions | 25, 50, and 100 μM |
Applications | The cell viability of HepG2 cells was significantly inhibited by the introduction of DAPT in a dose-dependent manner. Compared with the control, in the colony formation assay, the colony number was found to be significantly suppressed in the 50 and 100 μM DAPT groups |
Animal models | Eight-week-old Apoe–/– mice (B6.129P2) |
Preparation Method | Mini osmotic pumps containing AngII (1 μg/min/kg) were implanted subcutaneously. At day 14, mice were randomly divided into two groups. A group of mice was administered DAPT (10 mg/kg dissolved in 10% ethanol, 90% corn oil) three times a week for the next 28 days. |
Dosage form | 10 mg/kg, p.o. |
Applications | This 10 mg/kg concentration was effective in reducing the aneurysm formation in AngII-induced mouse model of AAA. |
| 文献引用 | |
| 产品描述 | DAPT (GSI-IX) is an orally active γ-secretase inhibitor with IC50s of 115 nM and 200 nM for total amyloid-β (Aβ) and Aβ42 produced in human primary neuronal cultures, respectively.[1] In vitro experiment it indicated that treatment with 10 μM DAPT significantly reduced the expression of Il6, Il12 and iNos at 6 and 12 h compared with vehicle at both low- and high-LPS stimulation.[2]In vitro, with 25, 50, and 100 μM DAPT in HepG2 cells significantly inhibited the proliferation and migration ability of HepG2 cells.[3]In vitro test shown it that treatment with 0, 25, 50 and 75 μM DAPT in CNE-2 cells, pre-treatment with DAPT enhanced the effect of cisplatin in a dose-dependent manner. However, the CNE-2 cells were treated with increasing concentrations of DAPT, and there was no obvious effect on cell survival.[5] In vivo efficacy study it shown that treatment with 100 mg/kg DAPT subcutaneously in PDAPP mice, after 3 h the peak level of DAPT were 490 ng/g in the brain, and levels greater than 100 ng/g were sustained throughout the first 18 h.[1]DAPT (10, 30 and 100 mg/kg; p.o.) reduced the cortical total Aβ in a dose-dependent manner with a 50% reduction occuring at 100 mg/kg dosing.[1]In vivo test indicated it that DAPT was administered intragastrically once daily for 28 days in ICR mice that can effectively in ameliorating Cd-induced multi-organ damage and cognitive impairment in mice, because of DAPT restored abnormal performance in the Y-maze, forced swimming and Morris water maze (MWM) tests.[4] References: |
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