| CAS NO: | 1405-10-3 |
| 包装 | 价格(元) |
| 10mM (in 1mL H2O) | 电议 |
| 10g | 电议 |
| Physical Appearance | A solid |
| Storage | Store at -20°C |
| M.Wt | 712.72 |
| Cas No. | 1405-10-3 |
| Formula | C23H46N6O13·H2SO4 |
| Solubility | ≥33.75 mg/mL in H2O; insoluble in DMSO; insoluble in EtOH |
| Chemical Name | (2R,3R,4R,5R,6R)-5-amino-2-(aminomethyl)-6-[(1R,2S,3S,4R,6S)-4,6-diamino-2-[(2S,3R,4R,5R)-4-[(3R,4R,5R,6S)-3-amino-6-(aminomethyl)-4,5-dihydroxyoxan-2-yl]oxy-3-hydroxy-5-(hydroxymethyl)oxolan-2-yl]oxy-3-hydroxycyclohexyl]oxyoxane-3,4-diol;sulfuric acid |
| Canonical SMILES | C1C(C(C(C(C1N)OC2C(C(C(C(O2)CN)O)O)N)OC3C(C(C(O3)CO)OC4C(C(C(C(O4)CN)O)O)N)O)O)N.OS(=O)(=O)O |
| 运输条件 | 蓝冰运输或根据您的需求运输。 |
| 一般建议 | 为了使其更好的溶解，请用37℃加热试管并在超声波水浴中震动片刻。不同厂家不同批次产品溶解度各有差异，仅做参考。若实验所需浓度过大至产品溶解极限，请添加助溶剂助溶或自行调整浓度。溶液形式一般不宜长期储存，请尽快用完。 |
Neomycin sulfate belongs to a kind of aminoglycoside antibiotics, which is a potent inhibitor of hammerhead ribozyme cleavage reaction with aK1 of 13.5 μM.
Neomycin prefers to interact with the ribozyme-substrate complex, which reduces the cleavage rate by stabilizing the ground state of the complex thus destabilizing the transition state of the cleavage process [1]. Neomycin could inhibit the binding of Tat protein to thetrans-activating region (TAR) element of HIV-1 RNA. It turns out that neomycin works as a noncompetitive inhibitor by binding to the Tat-TAR complex and increasing theKoff for dissociation of the peptide from the RNA [2].
Neomycin is the most potent aminoglycoside in stabilizing a DNA triple helix, mainly direct to TAT, and mixed base DNA triplexes, better than known DNA minor groove binders and polyamines. TAT is the preference of triplets stabilized by neomycin, but CGC(+) triplets could be accommodated by it [3]. Neomycin induces a concentration- and voltage-dependent partial block of both the cytosolic and luminal faces of the channels, which shows more appetencies of the luminal area of interaction than the cytosolic area. Dissociation constant (Kb(0)) respectively are 210.20 ± 22.80 and 589.70 ± 184.00 nM for luminal and cytosolic area when zero-voltage. Neomycin also exhibits voltage-dependent relief of block when holding potentials >+ 60 mv [4].
Reference:
[1] Stage T K, Hertel K J, Uhlenbeck O C. Inhibition of the hammerhead ribozyme by neomycin [J]. RNA, 1995, 1(1): 95-101.
[2] Wang S, Huber P W, Cui M, et al. Binding of Neomycin to the TAR Element of HIV-1 RNA Induces Dissociation of Tat Protein by an Allosteric Mechanism [J]. Biochemistry, 1998, 37(16): 5549–5557.
[3] Arya D P, Micovic L, Charles I, et al. Neomycin binding to Watson-Hoogsteen (W-H) DNA triplex groove: a model [J]. Journal of the American Chemical Society, 2003, 125(13): 3733-3744.
[4] Mead F, Williams A J. Block of the ryanodine receptor channel by neomycin is relieved at high holding potentials [J]. Biophysical Journal, 2002, 82(4): 1953-1963.
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