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Menadione
本产品不向个人销售，仅用作科学研究，不用于任何人体实验及非科研性质的动物实验。
Menadione图片
CAS NO:58-27-5
包装与价格：
包装价格(元)
10mM (in 1mL DMSO)电议
500mg电议
1g电议

产品介绍

化学性质

Physical AppearanceA solid
StorageStore at -20°C
M.Wt172.18
Cas No.58-27-5
FormulaC11H8O2
Solubilityinsoluble in H2O; ≥5.15 mg/mL in DMSO; ≥9.86 mg/mL in EtOH with ultrasonic
Chemical Name2-methylnaphthalene-1,4-dione
Canonical SMILESCC1=CC(=O)C2=CC=CC=C2C1=O
运输条件蓝冰运输或根据您的需求运输。
一般建议为了使其更好的溶解，请用37℃加热试管并在超声波水浴中震动片刻。不同厂家不同批次产品溶解度各有差异，仅做参考。若实验所需浓度过大至产品溶解极限，请添加助溶剂助溶或自行调整浓度。溶液形式一般不宜长期储存，请尽快用完。

资料参考

Menadione (vitamin K3), used as a nutritional supplement, is an inhibitor of mitochondrial DNA polymerase γ (pol γ), with an IC 50 value of 6 μM [1].

Pol γ is responsible for all aspects of mitochondrial DNA synthesis, including all replication, recombination of the mitochondrial genome, and repair of mitochondrial DNA damage [1].

In the extract of mitochondrion fraction from HCT116 (p53+/+ andp53–/–) cells, menadione at 30 μM inhibited pol γ by more than 80%. In HCT116 cells, 30 μM menadione also caused impairment of mitochondrial DNA replication and repair, and triggered a significant increase in reactive oxygen species (ROS), leading to apoptosis. At a lower concentration of 3 μM, menadione did not significantly increase the ROS level, but was able to effectively inhibit cancer cell proliferation, which could be reversed by supplementing glycolytic substrates [2].

In Emory mice, menadione at a low non-toxic dose of 0.12% (w/w), used as a dietary supplement for 10 to 12 weeks, caused early signs of cataract, such as prominent anterior suture, in 68% of the Emory mice [3].

References:

[1]. Mizushina Y, Yonezawa Y, Yoshida H. Selective inhibition of animal DNA polymerases by fat-soluble vitamins A, D, E and K and their related compounds. Current Enzyme Inhibition, 2007, 3(1): -.

[2]. Sasaki R, Suzuki Y, Yonezawa Y, et al. DNA polymerase gamma inhibition by vitamin K3 induces mitochondria-mediated cytotoxicity in human cancer cells. Cancer Science, 2008, 99(5): 1040-1048.

[3]. Bhuyan D K, Huang X, Kuriakose G, et al. Menadione-induced oxidative stress accelerates onset of Emory mouse cataract in vivo. Current eye research, 1997.

试验操作

Cell experiment:[1]

Cell lines

HCT116p53+/+andp53–/–cells

Reaction Conditions

3 or 30 μM menadione for 24 h incubation

Applications

Menadione at 30 μM inhibited DNA polymerase γ by more than 80%, caused impairment of mitochondrial DNA replication and repair, and induced a significant increase in reactive oxygen species (ROS), leading to apoptosis. At a lower concentration (3 μM), menadione did not cause a significant increase in ROS, but was able to effectively inhibit cell proliferation, which could be reversed by supplementing glycolytic substrates.

Animal experiment:[2]

Animal models

Four-week-old Emory mice

Dosage form

0.04%, 0.12% and 0.4% (w/w) menadione mixed with freshly ground Purina Rodent Lab Chow 5001

By oral route for 10 to 12 weeks

Applications

In Emory mice, menadione at a low non-toxic dose (0.12%, w/w), used as a dietary supplement for 10 to 12 weeks, caused early signs of cataract, such as prominent anterior suture, in 68% of the Emory mice.

Note

The technical data provided above is for reference only.

References:

1. Sasaki R, Suzuki Y, Yonezawa Y, et al. DNA polymerase gamma inhibition by vitamin K3 induces mitochondria-mediated cytotoxicity in human cancer cells. Cancer Science, 2008, 99(5): 1040-1048.

2. Bhuyan DK, Huang X, Kuriakose G, et al. Menadione-induced oxidative stress accelerates onset of Emory mouse cataract in vivo. Current eye research, 1997, 16(6): 519-526.