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Temozolomide(TMZ)
本产品不向个人销售,仅用作科学研究,不用于任何人体实验及非科研性质的动物实验。
Temozolomide(TMZ)图片
CAS NO:85622-93-1
包装:10mg, 50mg, 100mg, 500mg
包装与价格:
包装价格(元)
Free Sample (0.5-1 mg)电议
10mM*1mL in DMSO电议
10mg电议
50mg电议
100mg电议
500mg电议
1g电议

产品名称
NSC362856
CCRG81045
产品介绍

生物活性

Temozolomide (TMZ)是一种DNA损伤诱导剂。Temozolomide是一种甲基化剂,可穿过血脑屏障,适用于恶性胶质瘤和转移性黑色素瘤。


化学数据

分子量194.15
分子式C6H6N6O2
CAS号85622-93-1
纯度99.86%
溶解性(25°C)DMSO 18 mg/mL
储存和运输条件-20°C, protect from light, sealed
常温运输及临时存放

实验操作 来自于公开的文献,仅供相同实验参考(如实验材料、目的不同,请参考其他文献)

细胞实验
细胞系A2058, A375, M238 and M249 cell lines
方法Colony formation assay Depending on the cell line (and plating efficiency), 200–1000 cells were seeded into each well of a 6-well plate and treated as described in detail previously. In the case of primary melanoma cells, 1000 cells were seeded and let grow for 24 days in the presence or absence of drug treatment; because only small colonies formed, the stained colonies were counted under the microscope.Survival of melanoma cells after drug treatment. Five different melanoma cell lines (as indicated), as well as a culture of primary melanoma tissue cells (labeled ‘primary’) were exposed to increasing concentrations of TMZ (diamonds) or NEO212 (circles) for 48 hours, and long-term survival was determined via colony formation assay (CFA).
浓度0, 25, 50, 75, 100μM
处理时间48 h

动物实验
动物模型A375 cells subcutaneous tumor growth model
配制45% glycerol, 45% ethanol, 10% DMSO
剂量50 mg/kg
给药处理subcutaneous injection

不同实验动物依据体表面积的等效剂量转换表(数据来源于FDA指南)

小鼠大鼠豚鼠仓鼠
重量 (kg)0.020.151.80.40.0810
体表面积 (m2)0.0070.0250.150.050.020.5
Km系数36128520
动物 A (mg/kg) = 动物 B (mg/kg) × 动物 B的Km系数
动物 A的Km系数

例如,依据体表面积折算法,将化合物用于小鼠的剂量20 mg/kg 换算成大鼠的剂量,需要将20 mg/kg 乘以小鼠的Km系数(3),再除以大鼠的Km系数(6),得到化合物用于大鼠的等效剂量为10 mg/kg。


储备液配制

以下数据基于产品分子量,对于特殊产品,请参照COA中的储备液配制条件和说明进行操作。

Concentration / Solvent Volume / Mass1 mg5 mg10 mg
1 mM5.1507 mL25.7533 mL51.5066 mL
5 mM1.0301 mL5.1507 mL10.3013 mL
10 mM0.5151 mL2.5753 mL5.1507 mL