| CAS NO: | 110590-60-8 |
| 包装 | 价格(元) |
| 10mM (in 1mL DMSO) | 电议 |
| 1mg | 电议 |
| 5mg | 电议 |
| 10mg | 电议 |
| 25mg | 电议 |
| Physical Appearance | A solid |
| Storage | Store at -20°C |
| M.Wt | 967.06 |
| Cas No. | 110590-60-8 |
| Formula | C40H62N12O14S |
| Synonyms | H2N-Cys-Asp-Pro-Gly-Tyr-Ile-Gly-Ser-Arg-OH |
| Solubility | ≥15.53 mg/mL in H2O; ≥17.77 mg/mL in EtOH; ≥48.35 mg/mL in DMSO |
| Chemical Name | Laminin (925-933) |
| Canonical SMILES | CCC(C)C(C(=O)NCC(=O)NC(CO)C(=O)NC(CCCN=C(N)N)C(=O)O)NC(=O)C(CC1=CC=C(C=C1)O)NC(=O)CNC(=O)C2CCCN2C(=O)C(CC(=O)O)NC(=O)C(CS)N |
| 运输条件 | 蓝冰运输或根据您的需求运输。 |
| 一般建议 | 为了使其更好的溶解，请用37℃加热试管并在超声波水浴中震动片刻。不同厂家不同批次产品溶解度各有差异，仅做参考。若实验所需浓度过大至产品溶解极限，请添加助溶剂助溶或自行调整浓度。溶液形式一般不宜长期储存，请尽快用完。 |
层粘连蛋白（925-933）是一种来源于层粘连蛋白B1链925-933残基的多肽（Cys-Asp-Pro-Gly-Tyr-Ile-Gly-Ser-Arg），与层粘连蛋白受体结合。
层粘连蛋白属于细胞外基质糖蛋白家族，是基底膜的主要非胶原成分。它们参与多种生物学过程，包括细胞粘着、分化、迁移、信号通路、突起生长和癌转移。层粘连蛋白由3条不同的链组成，分别为层粘连蛋白α、β和γ（以前分别称为A、B1和B2）。
层粘连蛋白β1链有7个结构上不同的区域，这是与其它β链异构体共有的部分。层粘连蛋白β1在大多数产生基底膜的组织中表达，是组成层粘连蛋白1的3条链之一。层粘连蛋白1是从Engelbreth-Holm-Swarm（EHS）肿瘤中分离到的第一个层粘连蛋白。β1链中参与细胞附着、趋化作用和与层粘连蛋白受体结合的序列已被鉴定到，其具有抑制癌转移的能力。
Figure1 Structure of Laminin
Figure2 Formula of LamininB1 (925-933)
参考文献：
1.Ikonen J, Pikkarainen T, Savolainen ER, Tryggvason K (Feb 1989). "A Hpa I polymorphism in the human laminin B1 chain gene on 7q22".Nucleic Acids Res17(1): 473.
2.Roche KB, Moore JW, Surana RB, Wilson BE (May 1989). "Aortic root dilatation associated with partial trisomy 7(q31.2----qter)".Pediatr Cardiol10(1): 53–5.
3.Bonneau D, Huret JL, Godeau G, Couet D, Putterman M, Tanzer J, Babin P, Larregue M (Sep 1991). "Recurrent ctb(7)(q31.3) and possible laminin involvement in a neonatal cutis laxa with a Marfan phenotype".Hum Genet87(3): 317–9.
| Cell experiment:[1] | |
Cell lines | HT-1080 and CHO cells, as well as B16F10 murine melanoma cells |
Reaction Conditions | HT-1080 and CHO cells, as well as B16F10 murine melanoma cells |
Applications | Laminin (925-933) stimulated the attachment of HT-1080 and CHO cells to culture plates when used at concentrations of 100 and 300 μg/ml for 1 h incubation. In addition, laminin (925-933) (100 μg/ml; 5 h incubation) was found to be a chemoattractant for the B16F10 murine melanoma cells, showing about 30% of the maximal response observed with full-length laminin. Laminin (925-933) could compete with full-length laminin and inhibit chemotactic response of the cells to full-length laminin. |
Note | The technical data provided above is for reference only. |
References: 1. Graf J, Iwamoto Y, Sasaki M, et al. Identification of an amino acid sequence in laminin mediating cell attachment, chemotaxis, and receptor binding. Cell, 1987, 48(6): 989-996. | |
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