| 包装 | 价格(元) |
| 5mg | 电议 |
| 25mg | 电议 |
| Physical Appearance | A solid |
| Storage | Store at -20°C |
| M.Wt | 727.68 |
| Formula | C32H40F3N5O11 |
| Solubility | ≥72.8 mg/mL in DMSO; insoluble in EtOH; insoluble in H2O |
| Chemical Name | 10-(sec-butyl)-7-(2-carboxyethyl)-4-isopropyl-2,5,8,11-tetraoxo-13-((2-oxo-4-(trifluoromethyl)-2H-chromen-7-yl)carbamoyl)-3,6,9,12-tetraazapentadecan-15-oic acid |
| Canonical SMILES | O=C(O)CCC(NC(C(NC(C)=O)C(C)C)=O)C(NC(C(C)CC)C(NC(CC(O)=O)C(NC1=CC(O2)=C(C=C1)C(C(F)(F)F)=CC2=O)=O)=O)=O |
| 运输条件 | 蓝冰运输或根据您的需求运输。 |
| 一般建议 | 为了使其更好的溶解，请用37℃加热试管并在超声波水浴中震动片刻。不同厂家不同批次产品溶解度各有差异，仅做参考。若实验所需浓度过大至产品溶解极限，请添加助溶剂助溶或自行调整浓度。溶液形式一般不宜长期储存，请尽快用完。 |
Ac-VEID-AFC是一种荧光显色的caspase-6的底物(Km=30μM; Kcat/Km=168000 M-1sec-1)。也是其他相关的cysteine proteases的底物。这个序列是基于lamin A 的切割位点。在凋亡过程中，caspase-6 切割底物，释放可以被荧光探测的AFC（Ex/Em = 400/505）。在凋亡的细胞裂解物中，Ac-IETD-pNA可以用来鉴别和量化caspase-6活性，以及研究caspase-6 下游的信号通路。
References:
1. A combinatorial approach defines specificities of members of the caspase family and granzyme B. Functional relationships established for key mediators of apoptosis: N.A. Thornberry, et al.; J. Biol. Chem. 272, 17907 (1997)
2. Substrate specificities of caspase family proteases: R.V. Talanian, et al.; J. Biol. Chem. 272, 9677 (1997)
3. The Ced-3/interleukin 1beta converting enzyme-like homolog Mch6 and the lamin-cleaving enzyme Mch2alpha are substrates for the apoptotic mediator CPP32: S.M. Srinivasula, et al.; J. Biol. Chem. 271, 27099 (1996)
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