Brilliant Blue G

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Brilliant Blue G

本产品不向个人销售，仅用作科学研究，不用于任何人体实验及非科研性质的动物实验。

CAS NO:	6104-58-1
包装:	100g
市场价:	871元

产品介绍

化学性质

Storage	Store at RT
M.Wt	854
Cas No.	6104-58-1
Formula	C₄₇H₄₈N₃O₇S₂·Na
Synonyms	Acid Blue 90,CBBG,Coomassie Brilliant Blue G-250,NSC 328382
Solubility	≥21.35 mg/mL in DMSO; insoluble in H2O; insoluble in EtOH
Chemical Name	N-[4-[[4-[(4-ethoxyphenyl)amino]phenyl][4-[ethyl[(3-sulfophenyl)methyl]amino]-2-methylphenyl]methylene]-3-methyl-2,5-cyclohexadien-1-ylidene]-N-ethyl-3-sulfo-benzenemethanaminium, sodium salt
Canonical SMILES	CC1=CC(N(CC)CC2=CC=CC(S(=O)([O-])=O)=C2)=CC=C1/C(C3=CC=C(NC4=CC=C(OCC)C=C4)C=C3)=C5C=C/C(C=C\5C)=[N+](CC6=CC=CC(S(=O)([O-])=O)=C6)/CC.[Na+]
运输条件	蓝冰运输或根据您的需求运输。
一般建议	为了使其更好的溶解，请用37℃加热试管并在超声波水浴中震动片刻。不同厂家不同批次产品溶解度各有差异，仅做参考。若实验所需浓度过大至产品溶解极限，请添加助溶剂助溶或自行调整浓度。溶液形式一般不宜长期储存，请尽快用完。

资料参考

Coomassie Brilliant Blue G-250 is used for protein staining in SDS-PAGE, Blue Native PAGE, and the Bradford Method, with high band visibility. In the staining reaction, the Coomassie dye binds to proteins through ionic interactions between sulfonic acid groups and positive protein amine groups through Van der Waals attractions. The gels are soaked in dye and excess stain is then eluted with a solvent (“de-staining”). This treatment allows the visualization of protein bands.

Brilliant Blue G is also a selective inhibitor of the P2X purinoceptor channel P2X7 [1].

P2X receptors are membrane ion channels activated in response to the binding of extracellular ATP. Seven P2X subtypes have been identified. P2X receptors have been involved in in diverse patho- and physiological processes, such as the autonomic nervous system, afferent signalling, chronic pain, and in autocrine loops of endothelial and epithelial cells. The P2X7 receptor plays a prominent role in certain neurologic disorders, such as ischemia-reperfusion injury, Alzheimer's disease, spinal cord injury and sensory neuropathies [2].

In HEK293 cells heterologously expressing human P2X7 receptors, Brilliant Blue G noncompetitively inhibited rat and human P2X7 receptors with IC50 values of 10 and 200 nM, respectively. The IC50 values for inhibition of the other P2X receptors ranged from 2 to >30 μM; Brilliant Blue G inhibited the rat and human P2X4 receptors with the IC50 values of >10 and 3.2 μM [1]. Subretinal injection of BBG caused retinal cell degeneration at lower concentrations. Subretinal injection of BBG (0.25 mg/mL) provided satisfactory biocompatibility [3].

References:
[1] Jiang L H, Mackenzie A B, North R A, et al. Brilliant blue G selectively blocks ATP-gated rat P2X7 receptors[J]. Molecular pharmacology, 2000, 58(1): 82-88.
[2] Sperlágh B, Vizi E S, Wirkner K, et al. P2X 7 receptors in the nervous system[J]. Progress in neurobiology, 2006, 78(6): 327-346.
[3] Ueno A, Hisatomi T, Enaida H, et al. Biocompatibility of brilliant blue G in a rat model of subretinal injection[J]. Retina, 2007, 27(4): 499-504.