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NG-amino-L-Arginine(hydrochloride)
本产品不向个人销售,仅用作科学研究,不用于任何人体实验及非科研性质的动物实验。
NG-amino-L-Arginine(hydrochloride)图片
CAS NO:1031799-40-2
包装与价格:
包装价格(元)
10mg电议
25mg电议
50mg电议

产品介绍

化学性质

Physical AppearanceA crystalline solid
StorageStore at -20°C
M.Wt225.7
Cas No.1031799-40-2
FormulaC6H15N5O2·HCl
Solubility≤10mg/ml in PBS(pH7.2)
Chemical NameN5-(hydrazinyliminomethyl)-L-ornithine, monohydrochloride
Canonical SMILESNNC(NCCC[C@H](N)C(O)=O)=N.Cl
运输条件蓝冰运输或根据您的需求运输。
一般建议为了使其更好的溶解,请用37℃加热试管并在超声波水浴中震动片刻。不同厂家不同批次产品溶解度各有差异,仅做参考。若实验所需浓度过大至产品溶解极限,请添加助溶剂助溶或自行调整浓度。溶液形式一般不宜长期储存,请尽快用完。

资料参考

NG-amino-L-arginine, a novel structural analog of L-arginine, is an inhibitor of nitric oxide synthase (NOS) [1,2]. Nitric oxide synthases (NOSs) have been involved in catalyzing the production of nitric oxide (NO) from L-arginine. As an important cellular signaling molecule, NO has been implicated in modulating vascular tone, airway tone, insulinsecretion, and peristalsis. It has also been shown that NO is involved in angiogenesis and neural development and can function as a retrograde neurotransmitter [3].

In vitro: NG-amino-L-arginine potently and stereoselectively induced endothelium-dependent contraction. NG-amino-L-arginine caused concentration-dependent, competitive, and stereoselective antagonism of acetylcholine-elicited relaxation and cyclic GMP accumulation. NG-Amino-L-arginine was 100- to 300- fold more potent than NG-methyl-L-arginine [1]. NG-amino-L-arginine (100 μM) almost abolished endothelium-dependent relaxation induced by acetylcholine, but was rapidly restored by addition of 300 μM L-arginine. The maximal response to acetylcholine was inhibited by NG-amino-L-arginine in excess of 1 μM and was abolished by concentrations in the range of l0-30 μM [1]. NG-Amino-L-arginine inactivated the citrulline-forming activity of the nNOS, iNOS, and eNOS isoforms with the maximal inactivation rates of 0.35, 0.26, and 0.53 min-1 and Ki values of 0.3, 3, and 2.5 μM, respectively [2].

In vivo: In awake animal models of sepsis, treatment with NG-amino-L-arginine showed higher systemic and pulmonary vascular resistance indices and decreased heart rates, cardiac indices, oxygen delivery indices, and oxygen consumption indices when compared with controls [4]. NG-amino-L-arginine increased mortality rates after endotoxin challenge [4].

References:
[1] Fukuto J M, Wood K S, Byrns R E, et al.  NG-amino-L-arginine: a new potent antagonist of L-arginine-mediated endothelium-dependent relaxation[J]. Biochemical and biophysical research communications, 1990, 168(2): 458-465.
[2] Wolff D J, Lubeskie A.  Inactivation of Nitric Oxide Synthase Isoforms by Diaminoguanidine andN G-Amino-L-arginine[J]. Archives of biochemistry and biophysics, 1996, 325(2): 227-234.
[3] Frstermann U, Closs E I, Pollock J S, et al.  Nitric oxide synthase isozymes. Characterization, purification, molecular cloning, and functions[J]. Hypertension, 1994, 23(6 Pt 2): 1121-1131.
[4] Cobb J P, Natanson C, Hoffman W D, et al.  N omega-amino-L-arginine, an inhibitor of nitric oxide synthase, raises vascular resistance but increases mortality rates in awake canines challenged with endotoxin[J]. Journal of Experimental Medicine, 1992, 176(4): 1175-1182.