| 包装 | 价格(元) |
| 100ug (1mg/mL) | 电议 |
| 1mg (1mg/mL) | 电议 |
| 5x1mg (1mg/mL) | 电议 |
| mRNA Length | 1437 nucleotides |
| Concentration | 1 mg/mL |
| Buffer | 1 mM Sodium Citrate, pH 6.4 |
| Storage | -40°C or below |
| General tips | 请将其于冰上溶解,并小心防止RNase污染降解。尽可能避免反复冻融，不要涡旋震荡。首次使用时，将其轻柔离心并分成几份，可供单独使用。使用不含RNase的试剂和耗材，使用适当的无RNase技术。直至与转染试剂混合，才可加入含有血清的培养基中。 |
| Shipping Condition | 干冰运输 |
p53 is one of the most studied transcription factors. p53 can activate its downstream genes in response to carcinogenic signals, such as apoptosis promoting protein BAX, p53 upregulates apoptosis regulator PUMA, etc. p53 also acts as a cell cycle checkpoint protector to induce cell cycle arrest and participate in DNA replication and repair to protect genomic integrity.
Synthetic mRNA delivery of p53 tumor suppressor function to cancer cells enables combination drug therapy. EZ Cap(TM) Human p53 mRNA (ψUTP) is provided at a concentration of 1mg/ml. It is co-transcriptional capped which generates a cap 1 structure with high efficiency. Cap 1 structure is more ideal for mammalian systems and possesses higher transcription efficiency than Cap 0 structure (ARCA and mCap). ψUTP and poly (A) tail suppress RNA-mediated innate immune activation and increase the stability and lifetime of the mRNA in vitro and in vivo. Poly (A) tail also plays an important role in enhancing the efficiency of translation initiation.
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