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5-Ethynyl-2'-deoxyuridine(5-EdU)
本产品不向个人销售，仅用作科学研究，不用于任何人体实验及非科研性质的动物实验。
5-Ethynyl-2'-deoxyuridine(5-EdU)图片
CAS NO:61135-33-9
包装与价格：
包装价格(元)
100mg电议
1g电议

产品介绍

化学性质

Physical AppearanceSolution
StorageStore at -20°C
M.Wt252.22
Cas No.61135-33-9
FormulaC11H12N2O5
Solubility≥25.2 mg/mL in DMSO; insoluble in EtOH; ≥3.16 mg/mL in H2O with gentle warming and ultrasonic
Chemical Name5-ethynyl-1-((2R,4S,5R)-4-hydroxy-5-(hydroxymethyl)tetrahydrofuran-2-yl)pyrimidine-2,4(1H,3H)-dione
Canonical SMILESO=C1C(C#C)=CN([C@@H]2O[C@H](CO)[C@@H](O)C2)C(N1)=O
运输条件蓝冰运输或根据您的需求运输。
一般建议为了使其更好的溶解，请用37℃加热试管并在超声波水浴中震动片刻。不同厂家不同批次产品溶解度各有差异，仅做参考。若实验所需浓度过大至产品溶解极限，请添加助溶剂助溶或自行调整浓度。溶液形式一般不宜长期储存，请尽快用完。

描述

5-Ethynyl-2'-deoxyuridine (5-EdU) is a thymidine analog that can be incorporated into replicating DNA for detection of cell proliferation. 5-EdU can be used as a replacement for BrdU (5-Bromo-2'-deoxyuridine) to measure de novo DNA synthesis during the S-phase of the cell cycle through click chemistry. Click chemistry is a method of covalently coupling an azide with an alkyne. 5-EdU has an alkyne handle that can be detected using azide-containing fluorescent probes through a highly efficient click chemistry reaction [1][2]. In contrast to the use of BrdU, the new method does not require sample fixation or DNA denaturation. 5-EdU-labelled cells may be isolated to determine the transcript of cells, from neural cancer or tissues that have undergone division in vitro and in vivo[3].

References：

[1] Zeng C, Pan F, et al. Evaluation of 5-ethynyl-2'-deoxyuridine staining as a sensitive and reliable method for studying cell proliferation in the adult nervous system. Brain Res. 2010 Mar 10;1319:21-32.

[2] Cavanagh BL, Walker T, et al. Thymidine analogues for tracking DNA synthesis. Molecules. 2011 Sep 15;16(9):7980-93.

[3] Endaya BB, Lam PY, et al. Transcriptional profiling of dividing tumor cells detects intratumor heterogeneity linked to cell proliferation in a brain tumor model. Mol Oncol. 2016 Jan;10(1):126-37.