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L-NAME hydrochloride
本产品不向个人销售，仅用作科学研究，不用于任何人体实验及非科研性质的动物实验。
L-NAME hydrochloride图片
CAS NO:51298-62-5
包装与价格：
包装价格(元)
100mg电议
10g电议
25g电议

产品介绍

化学性质

Physical AppearanceA solid
StorageStore at -20°C
M.Wt269.7
Cas No.51298-62-5
FormulaC7H15N5O4·HCl
SynonymsL-NAME,L-NAME HCl
Solubility≥27 mg/mL in H2O; insoluble in EtOH; ≥23 mg/mL in DMSO
Chemical Namemethyl (2S)-2-amino-5-[[amino(nitramido)methylidene]amino]pentanoate;hydrochloride
Canonical SMILESCOC(=O)C(CCCN=C(N)N[N+](=O)[O-])N.Cl
运输条件蓝冰运输或根据您的需求运输。
一般建议为了使其更好的溶解，请用37℃加热试管并在超声波水浴中震动片刻。不同厂家不同批次产品溶解度各有差异，仅做参考。若实验所需浓度过大至产品溶解极限，请添加助溶剂助溶或自行调整浓度。溶液形式一般不宜长期储存，请尽快用完。

资料参考

L-NAME Hydrochloride is an inhibitor of nitric oxide synthase (NOS) with IC50 value of 70 μM [1].

NOS is an important enzyme involved in production of NO. NO, the smallest signaling molecule known, controls servoregulatory functions such as neurotransmission or vascular tone, regulates gene transcription and mRNA translation, as well as produces post-translational modifications of proteins [2].

At concentrations of 10-9~ 10-3M, L-NAME Hydrochloride inhibited NOS purified from rat brains in a dose dependent manner, with IC50 value of 70 μM [1]. In another study, L-NAME Hydrochloride at 0.1 ~ 100 mM caused concentration-dependent inhibition of the Ca2+-dependent endothelial NO synthase from porcine aortae. L-NAME Hydrochloride also caused an endothelium-dependent contraction and an inhibition of the endothelium-dependent relaxation induced by acetylcholine (ACh) in aortic rings [3].

In anaesthetized rats, L-NAME Hydrochloride (0.03 ~ 300 mg/kg, i.v.) induced a dose-dependent increase in mean systemic arterial blood pressure accompanied by bradycardia. L-NAME Hydrochloride (100 mg/kg, i.v.) significantly inhibited the hypotensive responses to ACh and bradykinin. The increase in blood pressure and bradycardia produced by L-NAME Hydrochloride could be reversed by L-arginine (30 ~ 100 mg/kg, i.v.) in a dose-dependent manner [3].

References:

[1]. Pfeiffer S, Leopold E, Schmidt K, et al. Inhibition of nitric oxide synthesis by NG-nitro-L-arginine methyl ester (L-NAME): requirement for bioactivation to the free acid, NG-nitro-L-arginine. British Journal of Pharmacology, 1996, 118(6): 1433-1440.

[2]. Forstermann U, Sessa W C. Nitric oxide synthases: regulation and function. European Heart Journal, 2012, 33(7): 829-837.

[3]. Rees D D, Palmer R M, Schulz R, et al. Characterization of three inhibitors of endothelial nitric oxide synthase in vitro and in vivo. British Journal of Pharmacology, 1990, 101(3): 746-752.

试验操作

Cell experiment:[1]

Cell lines

Bovine retinal endothelial cells (BRECs) and rat retinal müller cell line rMC-1

Reaction Conditions

1 mM L-NAME for 5 d incubation

Applications

L-NAME inhibited production of NO and prostaglandin E2 as well as expression of iNOS and COX-2 in rMC-1 cells exposed to 25 mM glucose. Moreover, L-NAME also reduced BREC and rMC-1 cell death in the high glucose condition.

Animal experiment:[2]

Animal models

Male Wistar rats, 200 ~ 300 g

Dosage form

0.03 ~ 300 mg/kg

Intravenous administration (i.v.)

Applications

L-NAME (0.03 ~ 300 mg/kg, i.v.) induced a dose-dependent increase in mean systemic arterial blood pressure accompanied by bradycardia. Furthermore, L-NAME (100 mg/kg, i.v.) inhibited significantly the hypotensive responses to acetylcholine and bradykinin. The increases in blood pressure and bradycardia produced by L-NAME were reversed by L-arginine (30 ~ 100 mg/kg, i.v.) in a dose-dependent manner. Therefore, L-NAME could be used to study the important role of NO synthesis in the maintenance of vascular tone and blood pressure.

Note

The technical data provided above is for reference only.

References:

1. Du Y, Sarthy VP, Kern TS. Interaction between NO and COX pathways in retinal cells exposed to elevated glucose and retina of diabetic rats. American Journal of Physiology-Regulatory, Integrative and Comparative Physiology, 2004, 287(4): R735-741.

2. Rees DD, Palmer RM, Schulz R, et al. Characterization of three inhibitors of endothelial nitric oxide synthase in vitro and in vivo. British Journal of Pharmacology, 1990, 101(3): 746-752.

生物活性

DescriptionL-NAME hydrochloride is a NO synthase inhibitor.
Targets      
IC50