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Cy3-UTP
本产品不向个人销售,仅用作科学研究,不用于任何人体实验及非科研性质的动物实验。
Cy3-UTP图片
包装与价格:
包装价格(元)
25ul (10mM)电议
5x25ul (10mM)电议

产品介绍

化学性质

Physical AppearanceSolution
StorageStore at -20°C or below
M.Wt1151.98 (free acid)
FormulaC43H56N5O22P3S2(free acid)
SynonymsAminoallyl-UTP - Cy3;5-(3-Aminoallyl)-uridine-5'-triphosphate; labeled with Cy3;Triethylammonium salt;Cyanine 3-AA-UTP;Cyanine 3-Aminoallyluridine-5'-Triphosphate;Cyanine 3-UTP
SolubilitySoluble in H2O
Chemical Name2-((1E,3E)-3-(1-(6-(((E)-3-(1-((2R,3R,4S,5R)-3,4-dihydroxy-5-(((hydroxy((hydroxy(phosphonooxy)phosphoryl)oxy)phosphoryl)oxy)methyl)tetrahydrofuran-2-yl)-2,4-dioxo-1,2,3,4-tetrahydropyrimidin-5-yl)allyl)amino)-6-oxohexyl)-3,3-dimethyl-5-sulfoindolin-2-ylid
Canonical SMILESO=S(C1=CC=C([N+](CC)=C(C2(C)C)/C=C/C=C(C(C)(C)C3=C4C=CC(S(O)(=O)=O)=C3)/N4CCCCCC(NC/C=C/C5=CN([C@H]6[C@H](O)[C@H](O)[C@@H](COP(OP(O)(OP(O)(O)=O)=O)(O)=O)O6)C(NC5=O)=O)=O)C2=C1)([O-])=O
运输条件蓝冰运输或根据您的需求运输。
一般建议为了使其更好的溶解,请用37℃加热试管并在超声波水浴中震动片刻。不同厂家不同批次产品溶解度各有差异,仅做参考。若实验所需浓度过大至产品溶解极限,请添加助溶剂助溶或自行调整浓度。溶液形式一般不宜长期储存,请尽快用完。

资料参考

Cy3-UTP (Cyanine 3-UTP) can replace UTP as a substrate for T7 RNA polymerase and generate labeled probes throughin vitrotranscription. The resulting assembled mRNA will emit orange fluorescence with the involvement of Cy3-UTPs. In addition, Cy3-labeled mRNA is readily visible under ultraviolet light without further staining after gel electrophoresis[1].

Probes generated by these methods are suitable for multicolor fluorescence analysis, specifically dual-color expression arrays in conjunction with cyanine 3-UTP .

Reference:

[1]Kim, H., Park, Y., & Lee, J. B. (2015). Self-assembled Messenger RNA Nanoparticles (mRNA-NPs) for Efficient Gene Expression. Scientific Reports, 5(1). doi:10.1038/srep12737