(Z-Ala-Ala-Ala-Ala)2Rh110

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CAS NO:

149695-85-2

包装与价格：

包装	价格(元)
5mg	电议
10mg	电议

产品介绍

化学性质

Physical Appearance	A crystalline solid
Storage	Store at -20°C
M.Wt	1167.2
Cas No.	149695-85-2
Formula	C₆₀H₆₆N₁₀O₁₅
Synonyms	(Z-Ala-Ala-Ala-Ala)2Rhodamine110\|bis-(CBZ-L-alanyl-L-arginine amide) Rhodamine 110
Solubility	≤2mg/ml in DMSO;2mg/ml in dimethyl formamide
Chemical Name	N-[(phenylmethoxy)carbonyl]-L-alanyl-L-alanyl-L-alanyl-N-[3-oxo-6’-[[N-[N-[N-[N-[(phenylmethoxy)carbonyl]-L-alanyl]-L-alanyl]-L-alanyl]-L-alanyl]amino]spiro[isobenzofuran-1(3H),9’-[9H
Canonical SMILES	O=C(O1)C2=CC=CC=C2C31C4=C(C=C(NC(C(C)NC(C(C)NC(C(C)NC(C(C)NC(OCC5=CC=CC=C5)=O)=O)=O)=O)=O)C=C4)OC6=C3C=CC(NC(C(C)NC(C(C)NC(C(C)NC(C(C)NC(OCC7=CC=CC=C7)=O)=O)=O)=O)=O)=C6
运输条件	蓝冰运输或根据您的需求运输。
一般建议	为了使其更好的溶解，请用37℃加热试管并在超声波水浴中震动片刻。不同厂家不同批次产品溶解度各有差异，仅做参考。若实验所需浓度过大至产品溶解极限，请添加助溶剂助溶或自行调整浓度。溶液形式一般不宜长期储存，请尽快用完。

资料参考

(Z-Ala-Ala-Ala-Ala)2Rh110 is a fluorogenic elastase substrate.

Elastase can break down elastin that is an elastic fibre that, together with collagen and can determine the mechanical properties of connective tissue. The neutrophil form breaks down the Outer membrane protein A of E. coli and other Gram-negative bacteria. In addition, elastase has the critical immunological role of breaking down Shigella virulence factors.

In vitro: (Z-Ala-Ala-Ala-Ala)2Rh110 has been identified as a very sensitive fluorogenic elastase substrate. The colorless and nonfluorescent Z-Ala-Ala-Ala-Ala)2Rh110 was found to be selectively cleaved by elastase to yield the highly fluorescent compound rhodamine 110, which exhibited excellent spectral properties matching the optimal detection window of most fluorescence instruments. After cleavage, Abs(max) is at 497 nm and Em(max) is at 520 nm for rhodamine 110 [1].

In vivo: Currently, there is no animal in vivo data reported.

Clinical trial: So far, no clinical study has been conducted.

Reference:
[1] A. F. Johnson, M. D. Struthers, K. B. Pierson, et al. Nonisotopic DNA detection system employing elastase and a fluorogenic rhodamine substrate. Analytical Chemistry 65, 2352-2359 (1993).