包装 | 价格(元) |
1mg | 电议 |
5mg | 电议 |
Cell lines | HepG2 cells |
Preparation Method | Aflatoxin B1 (100 μM) was treated at 85 kV with HVACP for 0, 2, 5, 10, and 20 min. HepG2 cells were exposed to HVACP-treated Aflatoxin B1 for 72 h and assessed for cell viability, caspase-3 activity, DNA fragmentation, and protein carbonyls for each treatment time. |
Reaction Conditions | 100 μM; 0, 2, 5, 10, and 20 min |
Applications | Cell viability, caspase-3 activity, DNA fragmentation levels, and protein carbonyls contents of HepG2 cells exposed to HVACP-treated Aflatoxin B1 after 20 min was not significantly different compared to non-exposed HepG2 cells. However, their contents were significantly higher in non-exposed cells compared to the other HVACP treatment times. |
Animal models | Male Wistar rats |
Dosage form | 10, 20, or 50 μg/kg; i.m. |
Preparation method | Male Wistar rats were injected intramuscularly with doses of 10, 20, or 50 μg Aflatoxin B1/kg body weight on alternate days from 45 to 100 days of age. |
Applications | Significant reductions in body weights, relative weights of reproductive organs, daily sperm production, epididymal sperm count, viable sperm, motile sperm, and hypoosmotic swelling-tail coiled sperm were observed. Significant decreases in testicular steroidogenic enzymes and serum testosterone levels were also observed indicating decreased steroidogenesis. |
产品描述 | Aflatoxin B1, as a class of carcinogenic mycotoxins produced by Aspergillus fungi, always lead to the development of hepatocellular carcinoma (HCC) in humans and animals.[1]Aflatoxin B1's toxicity includes acute toxicity, teratogenicity, mutagenicity and carcinogenicity. Moreover, DNA adduction, inflammation and oxidative stress caused by Aflatoxin B1 can also participate in the occurrence of cancer.[2] In vitro, AFB1 has inhibition against cell viability of Hek293 cells with IC50 of 32.60 μM.[3]In vitro experiment it shown that with 0, 10, 50, and 100 μM AFB1, maturation of oocytes was performed. At concentrations of 50 and 100 μM AFB1, the number of oocytes reaching the metaphase II stage reduced and the oocytes presented with lower intracellular levels of GSH. And intracellular ROS production in matured oocytes also reached the highest-level.[4] In vivo test it shown that treatment with 5 mg/kg Aflatoxin B1 intraperitoneally in chicks markedly boost ALT and AST levels (indicators of liver damage).[5]In vivo efficacy test it exhibited that Aflatoxin B1 impairs animal performance following a subacute or chronic exposure, the LD50 values vary from 0.3 mg/kg body weight in ducklings to 9.0 mg/kg body weight in mice and up to 17.9 mg/kg body weight in female rats.[6]Pregnant mice were treated with a high dose of 20 mg/kg Aflatoxin B1 intraperitoneally, there has peak absorption in blood after 15 min, which was delayed to 30 min after oral ingestion, suggesting aflatoxin's uptake from the proximal intestine was very rapid.[7]In vivo, at a high-dose 75 μg/kg, Aflatoxin B1 significantly shown an over 70% reduction in the levels of fecal short-chain fatty acids (SCFAs). Moreover, Rats were treated with 25 μg/kg Aflatoxin B1 orally caused a remarkable elevations of fecal cholic acid (2.18-fold), linoleic acid (cis-9, cis-12-18:2) (11.3-fold), pentadecanoic acid (15: 0) (3.68-fold), pyruvic acid (4.56-fold), and 3-phenyllactic acid (3.74-fold).[8] References: |