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Apocynin
本产品不向个人销售，仅用作科学研究，不用于任何人体实验及非科研性质的动物实验。
Apocynin图片
CAS NO:498-02-2
包装与价格：
包装价格(元)
10mM (in 1mL DMSO)电议
1g电议

产品介绍

化学性质

Physical AppearanceA solid
StorageStore at -20°C
M.Wt166.17
Cas No.498-02-2
FormulaC9H10O3
Solubility≥8.25 mg/mL in DMSO
Chemical Name1-(4-hydroxy-3-methoxyphenyl)ethanone
Canonical SMILESOC1=CC=C(C(C)=O)C=C1OC
运输条件蓝冰运输或根据您的需求运输。
一般建议为了使其更好的溶解，请用37℃加热试管并在超声波水浴中震动片刻。不同厂家不同批次产品溶解度各有差异，仅做参考。若实验所需浓度过大至产品溶解极限，请添加助溶剂助溶或自行调整浓度。溶液形式一般不宜长期储存，请尽快用完。

资料参考

Apocynin is a selective NADPH-oxidase inhibitor with IC50 value of 10 μM [1].

Apocynin is an intracellular inhibitor of the assembly of NADPH oxidase, concomitantly inhibiting reactive oxygen species (ROS) production, which contributes to various anti-inflammatory effects [1, 2].

In human neutrophils stimulated by serum-treated zymosan (STZ), Apocynin completely inhibited oxygen uptake at the concentration of 300 μM. Inhibition of the STZ-induced respiratory burst by Apocynin was also observed in human eosinophils, but not in human alveolar macrophages [2].

In a mouse model of carrageenan-induced pleurisy, intraperitoneal injection of Apocynin at the dose of 5 mg/kg reduced lung injury and neutrophil infiltration. Apocynin also significantly reduced NADPH oxidase activity in lung tissues [3]. In a mouse model of spinal cord injury, treatment with Apocynin (5 mg/kg, i.p.) 1 and 6 h after the trauma significantly decreased the degree of spinal cord inflammation and tissue injury. Moreover, Apocynin substantially ameliorated the loss of limb function [4].

References:

[1]. Stefanska J, Pawliczak R. Apocynin: molecular aptitudes. Mediators of Inflammation, 2008, 2008: 106507.

[2]. Stolk J, Hiltermann T J, Dijkman J H, et al. Characteristics of the inhibition of NADPH oxidase activation in neutrophils by apocynin, a methoxy-substituted catechol. American Journal of Respiratory Cell and Molecular Biology, 1994, 11(1): 95-102.

[3]. Impellizzeri D, Esposito E, Mazzon E, et al. Effect of apocynin, a NADPH oxidase inhibitor, on acute lung inflammation. Biochemical Pharmacology, 2011, 81(5): 636-648.

[4]. Impellizzeri D, Mazzon E, Esposito E, et al. Effect of Apocynin, an inhibitor of NADPH oxidase, in the inflammatory process induced by an experimental model of spinal cord injury. Free Radical Research, 2011, 45(2): 221-236.

试验操作

Cell experiment:[1]

Cell lines

Human neutrophils and eosinophils

Reaction Conditions

300 μM apocynin for 5 min incubation

Applications

In human neutrophils stimulated by serum-treated zymosan (STZ), apocynin completely inhibited oxygen uptake at the concentration of 300 μM. Inhibition of the STZ-induced respiratory burst by apocynin was also observed in human eosinophils. These properties suggest that apocynin is an intracellular inhibitor of the assembly of NADPH oxidase in neutrophils and eosinophils.

Animal experiment:[2]

Animal models

A mouse model of carrageenan-induced pleurisy

Dosage form

5 mg/kg

Injected intraperitoneally 30 min prior and 30 min after carrageenan administration

Applications

In a mouse model of carrageenan-induced pleurisy, intraperitoneal injection of apocynin at the dose of 5 mg/kg reduced lung injury and neutrophil infiltration. Apocynin also significantly reduced NADPH oxidase activity in lung tissues.

Note

The technical data provided above is for reference only.

References:

1. Stolk J, Hiltermann T J, Dijkman J H, et al. Characteristics of the inhibition of NADPH oxidase activation in neutrophils by apocynin, a methoxy-substituted catechol. American Journal of Respiratory Cell and Molecular Biology, 1994, 11(1): 95-102.

2. Impellizzeri D, Esposito E, Mazzon E, et al. Effect of apocynin, a NADPH oxidase inhibitor, on acute lung inflammation. Biochemical Pharmacology, 2011, 81(5): 636-648.