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Cy5,5 maleimide(non-sulfonated)
本产品不向个人销售，仅用作科学研究，不用于任何人体实验及非科研性质的动物实验。
Cy5,5 maleimide(non-sulfonated)图片
包装与价格：
包装价格(元)
5mg电议
25mg电议

产品介绍

化学性质

Physical AppearanceA solid
Storage24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate.
M.Wt741.36
FormulaC46H49ClN4O3
Solubility≥74.1 mg/mL in DMSO; insoluble in H2O; insoluble in EtOH
Chemical Name6-[(2E)-1,1-dimethyl-2-[(2E,4E)-5-(1,1,3-trimethylbenzo[e]indol-3-ium-2-yl)penta-2,4-dienylidene]benzo[e]indol-3-yl]-N-[2-(2,5-dioxopyrrol-1-yl)ethyl]hexanamide
Canonical SMILESCC(/C1=C\C=C\C=C\C(C(C)2C)=[N+](C3=C2C4=CC=CC=C4C=C3)CCCCC/C([O-])=N/CCN5C(C=CC5=O)=O)(C6=C(N1C)C=CC7=CC=CC=C76)C.Cl
运输条件蓝冰运输或根据您的需求运输。
一般建议为了使其更好的溶解，请用37℃加热试管并在超声波水浴中震动片刻。不同厂家不同批次产品溶解度各有差异，仅做参考。若实验所需浓度过大至产品溶解极限，请添加助溶剂助溶或自行调整浓度。溶液形式一般不宜长期储存，请尽快用完。

资料参考

Cy5.5 maleimide is a thiol reactive dye which is capable of selective labeling of proteins which contain sulfhydryl groups. Cy5.5 maleimide has near infrared emission that makes it suitable for bioimaging applications. This reagent can replace Alexa Fluor 680 and DyLight 680. For biomolecule labeling, the labeling reagent has low aqueous solubility, using of organic co-solvent to dissolve this molecular is necessary for efficient reaction. First, Cyanine dye should be dissolved in organic solvent and then added to a solution of biomolecule in appropriate aqueous buffer.

Cy5.5-NLP conjugate was synthesized by the NLP peptide and the Cy5.5-maleimide in 100 μL of anhydrous DMF, to which 10 μL of DIPEA was added. In the Her2/neu positive (SKBR-3 and BT474) or Her2/neunegative (MDA-MB-231) breast cancer cells, intracellular localization of Cy5.5-labeled peptide conjugates make the effects visible [1].

Reference:
[1]Cai, H. ; Singh, A.N.; Sun, X.; Peng, F. Synthesis and Characterization of Her2-NLP Peptide Conjugates Targeting Circulating Breast Cancer Cells: Cellular Uptake and Localization by Fluorescent Microscopic Imaging. Journal of Fluorescence, 2015, 25(1), 113–117.

Spectral properties

最大激发光波 (nm)684最大发射光波 (nm)710
消光系数 (M-1cm-1)209000量子产量0.2
CF2600.07CF2800.03