Estradiol (β-Estradiol) 是一种类固醇激素和主要的女性性激素。Estradiol 可上调人子宫内膜干细胞 (hEnSCs) 神经标志物的表达并促进其神经分化。Estradiol 可用于癌症、神经退行性疾病和神经组织工程的相关研究。
| 生物活性 | Estradiol (β-Estradiol) is a steroid hormone and the major female sex hormone. Estradiol can up-regulate the expression of neural markers of human endometrial stem cells (hEnSCs) and promote their neural differentiation. Estradiol can be used for the research of cancers, neurodegenerative diseases and neural tissue engineering[1][2]. |
| IC50& Target | Human Endogenous Metabolite |
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体外研究
(In Vitro) | Estradiol (10 nM, 7 days) induces neural differentiation and increased neurite branching of human endometrial stem cells (hEnSCs)[1].Estradiol (17β-estradiol, 10 nM, 7 days) increases the expression of neuron-like cell markers (Tuj-1, nestin and NF-H) in neural-like cells differentiated from hEnSCs[1].
Cell Differentiation Assay[1] | Cell Line: | Isolated human endometrial stem cells (hEnSCs) from human endometrial tissue | | Concentration: | 10 nM | | Incubation Time: | 7 days | | Result: | Increased the number of neurite processes including neural differentiation and neurite branching. |
Immunofluorescence[1] | Cell Line: | Isolated human endometrial stem cells (hEnSCs) from human endometrial tissue | | Concentration: | 10 nM | | Incubation Time: | 7 days | | Result: | Increased the percentage of neural marker (Tuj-1, nestin and NF-H)-positive cells of 62.2±1.3%, 71.5±4% and 51.2±1.5% respectively. |
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体内研究
(In Vivo) | Estradiol (1 nM, the hippocampal slices from FBN-ARO-KO mice) rescues long-term potentiation (LTP) amplitude[1].Estradiol (0.0167 mg, implanted s.c., FBN-ARO-KO mice) rescues the molecular and functional deficits in FBN-ARO-KO mice[1].
| Animal Model: | FBN-ARO-KO Mice[2] | | Dosage: | 1 nM | | Administration: | Treated for the hippocampal slices | | Result: | Rescued long-term potentiation (LTP) amplitude of both male and female mice. |
| Animal Model: | FBN-ARO-KO Mice[2] | | Dosage: | 0.0167 mg | | Administration: | Alzet minipumps with Estradiol (implanted s.c.), examined 7 days after minipump implantation. | | Result: | Restored hippocampal and cortical E2 levels to 93%, phosphorylation of AKT, ERK and CREB in the hippocampus and cortex to 90-95%, BDNF level to 80-90%, restored both synaptophysin and PSD95 in the forebrain.Rescued the spatial learning and memory defects. |
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| 运输条件 | Room temperature in continental US; may vary elsewhere. |
| 储存方式 | | Powder | -20°C | 3 years | | 4°C | 2 years | | In solvent | -80°C | 6 months | | -20°C | 1 month |
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| 溶解性数据 | In Vitro: DMSO : ≥ 50 mg/mL(183.57 mM) Ethanol : 20 mg/mL(73.43 mM;Need ultrasonic) *"≥" means soluble, but saturation unknown. 配制储备液 | 1 mM | 3.6713 mL | 18.3567 mL | 36.7134 mL | | 5 mM | 0.7343 mL | 3.6713 mL | 7.3427 mL | | 10 mM | 0.3671 mL | 1.8357 mL | 3.6713 mL |
*请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
储备液的保存方式和期限：-80℃, 6 months; -20℃, 1 month。-80℃ 储存时，请在 6 个月内使用，-20℃ 储存时，请在 1 个月内使用。 In Vivo: 请根据您的实验动物和给药方式选择适当的溶解方案。以下溶解方案都请先按照In Vitro方式配制澄清的储备液，再依次添加助溶剂： ——为保证实验结果的可靠性，澄清的储备液可以根据储存条件，适当保存；体内实验的工作液，建议您现用现配，当天使用；
以下溶剂前显示的百
分比是指该溶剂在您配制终溶液中的体积占比；如在配制过程中出现沉淀、析出现象，可以通过加热和/或超声的方式助溶 1. 请依序添加每种溶剂： corn oil Solubility: 12.5 mg/mL (45.89 mM); Suspended solution; Need ultrasonic 2. 请依序添加每种溶剂： 10% DMSO 40%PEG300 5%Tween-80 45% saline Solubility: ≥ 2.5 mg/mL (9.18 mM); Clear solution
此方案可获得 ≥ 2.5 mg/mL (9.18 mM，饱和度未知) 的澄清溶液。 以 1 mL 工作液为例，取 100 μL 25.0 mg/mL 的澄清 DMSO 储备液加到 400 μL PEG300 中，混合均匀；向上述体系中加入50 μL Tween-80，混合均匀；然后继续加入 450 μL生理盐水定容至 1 mL。 3. 请依序添加每种溶剂： 5% DMSO 40%PEG300 5%Tween-80 50% saline Solubility: 2.5 mg/mL (9.18 mM); Suspended solution; Need ultrasonic 4. 请依序添加每种溶剂： 5% DMSO 95% (20%SBE-β-CDin saline) Solubility: ≥ 2.5 mg/mL (9.18 mM); Clear solution 5. 请依序添加每种溶剂： 10% DMSO 90% (20%SBE-β-CDin saline) Solubility: ≥ 2.08 mg/mL (7.64 mM); Clear solution
此方案可获得 ≥ 2.08 mg/mL (7.64 mM，饱和度未知) 的澄清溶液。 以 1 mL 工作液为例，取 100 μL 20.8 mg/mL 的澄清 DMSO 储备液加到 900 μL20% 的 SBE-β-CD 生理盐水水溶液中，混合均匀。 6. 请依序添加每种溶剂： 10% DMSO 90%corn oil Solubility: ≥ 2.08 mg/mL (7.64 mM); Clear solution
此方案可获得 ≥ 2.08 mg/mL (7.64 mM，饱和度未知) 的澄清溶液，此方案不适用于实验周期在半个月以上的实验。 以 1 mL 工作液为例，取 100 μL 20.8 mg/mL 的澄清 DMSO 储备液加到 900 μL玉米油中，混合均匀。 *以上所有助溶剂都可在本网站选购。
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