NHC-diphosphate is an active phosphorylated intracellularmetabolite of β-d-N4-Hydroxycytidine (NHC) (HY-125033) as a diphosphate form^[1]. NHC is a pyrimidine ribonucleoside and behaves as a potentanti-virus agent. NHC effectively inhibits the replication ofvenezuelan equine encephalitisvirus (VEEV),Chikungunyavirus (CHIKV) andhepatitisC virus (HCV)^[1].

Huh-7 cells are incubated with (10-50 μM; 4 h) NHC or a McGuigan phosphoramidate prodrug of NHC.
Intracellular levels of the parental compounds and phosphorylated metabolites are measured using LC-MS/MS. Small amounts of NHC-monophosphate (MP) and NHC-diphosphate (DP; HY-135867D) can be observed, while NHC-triphosphate remains the most abundant metabolite.^[1].

419.18

Solid

C₉H₁₅N₃O₁₂P₂

39023-73-9

Room temperature in continental US; may vary elsewhere.

-20°C, protect from light, stored under nitrogen

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen)

H₂O : 160 mg/mL(381.70 mM;Need ultrasonic)

请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
储备液的保存方式和期限：-80℃, 6 months; -20℃, 1 month (protect from light, stored under nitrogen)。-80℃ 储存时，请在 6 个月内使用，-20℃ 储存时，请在 1 个月内使用。