Azvudine (RO-0622) hydrochloride is a potentnucleosidereverse transcriptaseinhibitor (NRTI), with antiviral activity onHIV,HBVandHCV. Azvudine hydrochloride exerts highly potent inhibition onHIV-1(EC₅₀s ranging from 0.03 to 6.92 nM) andHIV-2(EC₅₀s ranging from 0.018 to 0.025 nM). Azvudine hydrochloride inhibits NRTI-resistant viral strains^[1].

Azvudine (RO-0622) hydrochloride displays strong inhibition on wild-type HIV-1_IIIBand HIV-1_RFwith an EC₅₀ranging from 30 to 110 pM. The EC₅₀values of Azvudine hydrochloride against HIV-1_KM018, HIV-1_TC-1and HIV-1_{WAN T69N}are 6.92, 0.34 and 0.45 nM, respectively. Azvudine is sensitive to NRTIs-resistant strain HIV-1_74V, PIs-resistant strains HIV-1_{L10R/M46I/L63P/V82T/I84V}and HIV-1_{RF V82F/184V}, and FIs-resistant strain pNL4-3_{gp41 (36G) V38A/N42T}. The EC₅₀values of Azvudine hydrochloride against these resistant strains are 0.11, 0.14, 0.37 and 0.36 nM respectively^[1].

322.68

Solid

C₉H₁₂ClFN₆O₄

1333126-31-0

Room temperature in continental US; may vary elsewhere.

4°C, sealed storage, away from moisture

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

H₂O : 125 mg/mL(387.38 mM;Need ultrasonic)

请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
储备液的保存方式和期限：-80℃, 6 months; -20℃, 1 month (sealed storage, away from moisture)。-80℃ 储存时，请在 6 个月内使用，-20℃ 储存时，请在 1 个月内使用。

请根据您的实验动物和给药方式选择适当的溶解方案。以下溶解方案都请先按照In Vitro方式配制澄清的储备液，再依次添加助溶剂：

——为保证实验结果的可靠性，澄清的储备液可以根据储存条件，适当保存；体内实验的工作液，建议您现用现配，当天使用； 以下溶剂前显示的百
分比是指该溶剂在您配制终溶液中的体积占比；如在配制过程中出现沉淀、析出现象，可以通过加热和/或超声的方式助溶

• 1.

  请依序添加每种溶剂： PBS

  Solubility: 14.29 mg/mL (44.29 mM); Clear solution; Need ultrasonic and warming and heat to 60℃