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Berberine chloride
本产品不向个人销售，仅用作科学研究，不用于任何人体实验及非科研性质的动物实验。
Berberine chloride图片
CAS NO:633-65-8
包装与价格：
包装价格(元)
10 mM * 1 mL in DMSO电议
100mg电议
1 g电议
5 g电议
10 g电议
50 g电议

产品名称
盐酸小檗碱
Natural Yellow 18 chloride
产品介绍
Berberine chloride 是一种生物碱，常用作抗生素。Berberine chloride 诱导活性氧 (ROS) 生成并抑制 DNA 拓扑异构酶 (topoisomerase)。抗肿瘤特性。
生物活性

Berberine chloride is an alkaloid that acts as anantibiotic. Berberine chloride inducesreactive oxygen species(ROS) generation and inhibitsDNAtopoisomerase. Antineoplastic properties[1].

IC50& Target

ROS[1]
DNA topoisomerase[1]

体外研究
(In Vitro)

Berberine (1.25-160 μM; 72 hours) has potential inhibitory effects on the proliferation of four colorectal carcinoma cell lines LoVo, HCT116, SW480, and HT-29[1].
Berberine (1.25-160 μM; 24-72 hours) induces a time- and dose-dependent inhibition of LoVo cell growth[1].
LoVo cells are exposure to Berberine (10-80 μM) for 24 h. Cell cycle analysis of 40 μM Berberine-treated LoVo cells by flow cytometry shows accumulation of cells in the G2/M phase[1].
Berberine (10-80 μM) suppresses cyclin B1, cdc2 and cdc25c protein expression after 24 h, especially at the dose of 80.0 μM[1].

Cell Proliferation Assay[1]

Cell Line:Four colorectal carcinoma cell lines LoVo, HCT116, SW480, and HT-29
Concentration:1.25, 2.5, 5, 10, 20, 40, 80, and 160 μM
Incubation Time:72 hours
Result:Inhibited the proliferation of four cell lines. The IC50ranged from 40.8±4.1 μM (LoVo) to 98.6±2.9 μM (HCT116).

Cell Proliferation Assay[1]

Cell Line:Colorectal carcinoma cell lines LoVo
Concentration:1.25, 2.5, 5, 10, 20, 40, 80, and 160 μM
Incubation Time:24, 48, 72 hours
Result:Induced a time- and dose-dependent inhibition of cell growth. By 72 h, 160.0 μM induced 71.1±1.9 % growth inhibitions in LoVo cells.

Cell Cycle Analysis[1]

Cell Line:LoVo cells
Concentration:0, 10, 20, 40, or 80 μM
Incubation Time:24 hours
Result:Exposure to 40.0 μM induced G2/M-phase cell cycle arrest, an increase in the G2/M-phase population and a progressive decline in the G1 population.

Western Blot Analysis[1]

Cell Line:LoVo cells
Concentration:10, 20, 40, or 80 μM
Incubation Time:24 hours
Result:Suppressed cyclin B1, cdc2 and cdc25c protein expression.
体内研究
(In Vivo)

Berberine (10, 30, or 50 mg/kg/day; gastrointestinal gavage; for 10 consecutive days) inhibits the growth of human colorectal adenocarcinoma in vivo. Berberine at doses of 30 and 50 mg/kg/day taken by gastrointestinal gavage shows inhibitory rates of 33.1% and 45.3% on the human colorectal adenocarcinoma xenograft growth in nude mice[1].

Animal Model:5-week-old BALB/c nu/nu mice with human colorectal adenocarcinoma LoVo xenografts[1]
Dosage:10, 30, or 50 mg/kg/day
Administration:Gastrointestinal gavage; for 10 consecutive days
Result:Showed inhibitory rates of 33.1 % and 45.3 % at doses of 30 and 50 mg/kg/day.
Clinical Trial
分子量

371.81

性状

Solid

Formula

C20H18ClNO4

CAS 号

633-65-8

中文名称

盐酸小檗碱；盐酸黄连素；盐酸黄莲素

结构分类
  • Alkaloids
  • Isoquinoline Alkaloids
来源
  • Plants
  • Rutaceae
  • Phellodendron amurenseRupr.
运输条件

Room temperature in continental US; may vary elsewhere.

储存方式

4°C, sealed storage, away from moisture

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

溶解性数据
In Vitro: 

DMSO : 12.5 mg/mL(33.62 mM;Need ultrasonic)

H2O :< 0.1 mg/mL (ultrasonic)(insoluble)

配制储备液
浓度溶剂体积质量1 mg5 mg10 mg
1 mM2.6895 mL13.4477 mL26.8955 mL
5 mM0.5379 mL2.6895 mL5.3791 mL
10 mM0.2690 mL1.3448 mL2.6895 mL
*

请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
储备液的保存方式和期限：-80℃, 6 months; -20℃, 1 month (sealed storage, away from moisture)。-80℃ 储存时，请在 6 个月内使用，-20℃ 储存时，请在 1 个月内使用。

In Vivo:

请根据您的实验动物和给药方式选择适当的溶解方案。以下溶解方案都请先按照In Vitro方式配制澄清的储备液，再依次添加助溶剂：

——为保证实验结果的可靠性，澄清的储备液可以根据储存条件，适当保存；体内实验的工作液，建议您现用现配，当天使用； 以下溶剂前显示的百
分比是指该溶剂在您配制终溶液中的体积占比；如在配制过程中出现沉淀、析出现象，可以通过加热和/或超声的方式助溶

  • 1.

    请依序添加每种溶剂： 0.5%CMC-Na/saline water

    Solubility: 11 mg/mL (29.59 mM); Suspended solution; Need ultrasonic

  • 2.

    请依序添加每种溶剂： PBS

    Solubility: 10 mg/mL (26.90 mM); Suspended solution; Need ultrasonic

  • 3.

    请依序添加每种溶剂： 10% DMSO    40%PEG300   5%Tween-80   45% saline

    Solubility: ≥ 1.25 mg/mL (3.36 mM); Clear solution

    此方案可获得 ≥ 1.25 mg/mL (3.36 mM，饱和度未知) 的澄清溶液。

    以 1 mL 工作液为例，取 100 μL 12.5 mg/mL 的澄清 DMSO 储备液加到 400 μL PEG300 中，混合均匀；向上述体系中加入50 μL Tween-80，混合均匀；然后继续加入 450 μL生理盐水定容至 1 mL。

    将 0.9 g 氯化钠，完全溶解于 100 mL ddH2O 中，得到澄清透明的生理盐水溶液
  • 4.

    请依序添加每种溶剂： 10% DMSO    90% (20%SBE-β-CDin saline)

    Solubility: ≥ 1.25 mg/mL (3.36 mM); Clear solution

    此方案可获得 ≥ 1.25 mg/mL (3.36 mM，饱和度未知) 的澄清溶液。

    以 1 mL 工作液为例，取 100 μL 12.5 mg/mL 的澄清 DMSO 储备液加到 900 μL20% 的 SBE-β-CD 生理盐水水溶液中，混合均匀。

    将 2 g 磺丁基醚 β-环糊精加入 5 mL 生理盐水中，再用生理盐水定容至 10 mL，完全溶解，澄清透明
*以上所有助溶剂都可在本网站选购。