| 生物活性 | Amentoflavone (Didemethyl-ginkgetin) is a potent and orally activeGABA(A)negative modulator. Amentoflavone also shows anti-inflammatory, antioxidative, anti-viral, anti-tumor, anti-radiation, anti-fungal, antibacterial activity. Amentoflavone inducesapoptosisand cell cycle arrest at sub-G1 phase[1][2][3][4]. |
体外研究
(In Vitro) | Amentoflavone (1-60 μM) inhibits the production of nitric oxide in a concentration-dependent manner in RAW 264.7 cells[2].
Amentoflavone (50-200 μM) inhibits the viability of U-87 MG cells with IC50value of 100 μM at 48 h[3].
Amentoflavone (0, 50, 100 μM; 48 h) induces apoptosis and cell cycle arrest at sub-G1 phase[3].
Amentoflavone (0, 50, 100 μM; 48 h) inhibits NF-qB activation and decreases the expression of MCL1 and C-FLIP protein in U-87 MG cells[3].
Amentoflavone (0-32 μg/ml) shows antibacterial activity with MICs of 8, 4, 32, 8, 16, 8 μg/ml forE. faeciumATCC 19434,S. aureusATCC 25923,S. mutansATCC 3065,E. coliO-157 ATCC 25922,E. coliATCC 43895,P. aeruginosaATCC 27853, respectively[4].
Cell Viability Assay[3] | Cell Line: | U-87 MG cells | | Concentration: | 0, 50, 75, 100, 200 μM | | Incubation Time: | 48 h | | Result: | Significantly inhibited the viability of U-87 MG cells by 23-71% with an IC50value of 100 μM at 48 h. |
Apoptosis Analysis[3] | Cell Line: | U-87 MG cells | | Concentration: | 0, 50, 100 μM | | Incubation Time: | 48 h | | Result: | Significantly induced the accumulation of cells in the sub-G1 population and increased the level of active caspase-3 by 14-52% and 24-42%, respectively, and significantly triggered the loss of Ψm and the expression of active caspase-8 by 23-53% and 25-50%, respectively. |
Western Blot Analysis[3] | Cell Line: | U-87 MG cells | | Concentration: | 0, 50, 100 μM | | Incubation Time: | 48 h | | Result: | Significantly reduced NF-qB activation in a dose-dependent manner by 25-87% and reduced protein expression of MCL1 and C-FLIP by 50-80% and 38-57%, respectively. |
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体内研究
(In Vivo) | Amentoflavone (25 mg/kg; p.o.; once a day for 3 consecutive days) shows neuroprotective role in epilepsy via anti-inflammatory effects in mouse[1].
| Animal Model: | 5-6 weeks, 28-32 g, kunming mice[1] | | Dosage: | 25 mg/kg | | Administration: | P.o.; once a day for 3 consecutive days | | Result: | Inhibited activation and nuclear translocation of NF-κB subunits p65, decreased IL-6 and IL-1β production and significantly decreased NO and prostaglandin E2 production. |
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| 来源 | - Plants
- Selaginellaceae
- Selaginella tamariscina(P. Beauv.) Spring
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| 运输条件 | Room temperature in continental US; may vary elsewhere. |
| 储存方式 | | Powder | -20°C | 3 years | | 4°C | 2 years | | In solvent | -80°C | 6 months | | -20°C | 1 month |
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| 溶解性数据 | In Vitro: DMSO : 125 mg/mL(232.14 mM;Need ultrasonic) 配制储备液 | 1 mM | 1.8571 mL | 9.2857 mL | 18.5715 mL | | 5 mM | 0.3714 mL | 1.8571 mL | 3.7143 mL | | 10 mM | 0.1857 mL | 0.9286 mL | 1.8571 mL |
*请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
储备液的保存方式和期限：-80℃, 6 months; -20℃, 1 month。-80℃ 储存时，请在 6 个月内使用，-20℃ 储存时，请在 1 个月内使用。 In Vivo: 请根据您的实验动物和给药方式选择适当的溶解方案。以下溶解方案都请先按照In Vitro方式配制澄清的储备液，再依次添加助溶剂： ——为保证实验结果的可靠性，澄清的储备液可以根据储存条件，适当保存；体内实验的工作液，建议您现用现配，当天使用；
以下溶剂前显示的百
分比是指该溶剂在您配制终溶液中的体积占比；如在配制过程中出现沉淀、析出现象，可以通过加热和/或超声的方式助溶 1. 请依序添加每种溶剂： 10% DMSO 40%PEG300 5%Tween-80 45% saline Solubility: ≥ 2.5 mg/mL (4.64 mM); Clear solution
此方案可获得 ≥ 2.5 mg/mL (4.64 mM，饱和度未知) 的澄清溶液。 以 1 mL 工作液为例，取 100 μL 25.0 mg/mL 的澄清 DMSO 储备液加到 400 μL PEG300 中，混合均匀；向上述体系中加入50 μL Tween-80，混合均匀；然后继续加入 450 μL生理盐水定容至 1 mL。 2. 请依序添加每种溶剂： 10% DMSO 90% (20%SBE-β-CDin saline) Solubility: 2.5 mg/mL (4.64 mM); Suspended solution; Need ultrasonic
此方案可获得 2.5 mg/mL (4.64 mM) 的均匀悬浊液，悬浊液可用于口服和腹腔注射。 以 1 mL 工作液为例，取 100 μL 25.0 mg/mL 的澄清 DMSO 储备液加到 900 μL20% 的 SBE-β-CD 生理盐水水溶液中，混合均匀。 *以上所有助溶剂都可在本网站选购。
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