Apostatin-1

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Apostatin-1

本产品不向个人销售，仅用作科学研究，不用于任何人体实验及非科研性质的动物实验。

CAS NO:

2559703-06-7

包装与价格：

包装	价格(元)
10 mM * 1 mL in DMSO	电议
5mg	电议
10mg	电议
25mg	电议
50mg	电议
100mg	电议
200mg	电议
500mg	电议

产品名称

Apt-1

产品介绍

Apostatin-1 (Apt-1) 是一种有效的TRADD抑制剂。Apostatin-1 可与TRADD-N结合 (K_D=2.17 μM)，破坏其与 TRADD-C 和 TRAF2 的结合。Apostatin-1 调节RIPK1和beclin 1的泛素化。Apostatin-1 通过激活突变 tau、α-synuclein 或 huntingtin 积累的细胞中的自噬 (autophagy)，阻断凋亡 (apoptosis)，并恢复细胞内稳态。

生物活性

Apostatin-1 (Apt-1) is a potentTRADDinhibitor. Apostatin-1 can bind withTRADD-N(K_D=2.17 μM), disrupting its binding to both TRADD-C and TRAF2. Apostatin-1 modulates the ubiquitination ofRIPK1andbeclin 1. Apostatin-1 blocksapoptosisand restores cellular homeostasis by activatingautophagyin cells with accumulated mutant tau,α-synuclein, orhuntingtin^[1].

体外研究
(In Vitro)

Apostatin-1 inhibits Velcade (Bortezomib, HY-134050)-induced apoptosis and RIPK1-dependent apoptosis (RDA) and necroptosis, with an IC₅₀of 0.97 μM^[1].
Apostatin-1 (10 μM, 6 h) effectively induces autophagy and the degradation of long-lived proteins^[1].

Western Blot Analysis^[1]

Cell Line:	SH-SY5Y, HeLa, HT-29, and Jurkat cells
Concentration:	10 μM
Incubation Time:	6 h
Result:	Effectively induced autophagy by LC3 II induction and p62 reduction.

体内研究
(In Vivo)

Apostatin-1 (20 mg/kg, IP, once) inhibits inflammatory responses, and increases survival of systemic inflammation mouse model^[1].

Animal Model:	Wild-type mice (n = 10, male, 8 weeks of age, systemic inflammation mouse model)^[1]
Dosage:	20 mg/kg
Administration:	IP, once
Result:	Reduced expression of the TNF-induced inflammatory target gene products, NOS and COXII27, and of inflammatory cytokines in cells stimulated with pathogen-associated molecular patterns, including interferon γ (IFNγ), lipopolysaccharide (LPS), Pam3CSK4 (a synthetic bacterial lipopeptide), and muramyl dipeptide (MDP). Showed increased survival following intravenous delivery of TNF, a mouse model of systemic inflammation.

分子量

345.50

性状

Solid

Formula

C₁₉H₂₇N₃OS

CAS 号

2559703-06-7

运输条件

Room temperature in continental US; may vary elsewhere.

储存方式

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	6 months
	-20°C	1 month

溶解性数据

In Vitro:

DMSO : 83.33 mg/mL(241.19 mM;ultrasonic and warming and adjust pH to 4 with HCl and heat to 80℃)

配制储备液

浓度溶剂体积质量

1 mg

5 mg

10 mg

1 mM	2.8944 mL	14.4718 mL	28.9436 mL
5 mM	0.5789 mL	2.8944 mL	5.7887 mL
10 mM	0.2894 mL	1.4472 mL	2.8944 mL

请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
储备液的保存方式和期限：-80℃, 6 months; -20℃, 1 month。-80℃ 储存时，请在 6 个月内使用，-20℃ 储存时，请在 1 个月内使用。