NecroIr2 is an iridium(III) complex, serves asnecroptosisinducers inCisplatin(HY-17394)-resistant lungcancercells (A549R). NecroIr2 selectively accumulates in mitochondria, leading to oxidative stress and loss of mitochondrial membrane potential (MMP). NecroIr2 activates receptor-interactingserine-threonine kinase 3(RIPK3) andmixed lineage kinasedomain-like pseudokinase(MLKL), and regulatesCDK4expression^[1].

NecroIr2 (2 μM; 1-2 d) exerts subcellular distribution over 90% accumulated in mitochondria in non-labelled A549R and CFSE labelled L02 cells^[1].
NecroIr2 (1.5 μM and 3 μM; 24 h) results ROS generation increasing and loss of mitochondrial membrane potential^[1].
NecroIr2 (1.5 μM and 3 μM; 24 h) activates necroptosis proteins, increases the phosphorylation of RIPK1 and RIPK3^[1].
NecroIr2 (0.75 μM and 1.5 μM; 24 h) induces necroptosis by arresting cell cycle at G0/G1^[1].
NecroIr2 (0.375-1.5 μM; 24 h) inhibits A549R cells proliferation^[1].

926.44

Solid

C₄₆H₃₀ClIrN₆O₂^-

Room temperature in continental US; may vary elsewhere.

4°C, sealed storage, away from moisture and light

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)

DMSO : 50 mg/mL(53.97 mM;Need ultrasonic)

请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
储备液的保存方式和期限：-80℃, 6 months; -20℃, 1 month (sealed storage, away from moisture and light)。-80℃ 储存时，请在 6 个月内使用，-20℃ 储存时，请在 1 个月内使用。