Patritumab (Human Anti-ERBB3 Recombinant Antibody) 是针对ERBB3的中和性单克隆抗体。Patritumab与 Cetuximab (HY-P9905) 具有协同作用，可有效抑制EGFR，HER2，HER3，ERK和AKT的磷酸化。Patritumab 也能诱导细胞凋亡 (apoptosis)，也抑制胰腺、非小细胞肺癌和结直肠癌异种移植物肿瘤的生长。
| 生物活性 | Patritumab (Human Anti-ERBB3 Recombinant Antibody) is a neutralizing monoclonal antibody toERBB3. Patritumab shows a synergy withCetuximab(HY-P9905), potently inhibits the phosphorylation ofEGFR,HER2,HER3,ERK, andAKT. Patritumab also induces cellapoptosisand suppresses the growth of pancreatic, non-small cell lungcancer, and colorectalcancerxenograft tumors[1]. |
体外研究
(In Vitro) | Patritumab targets to the extracellular domain (ECD) of HER3 and (10 μg/mL; 5 d) induces DiFi-HRG4 cells apoptosis[1].
Patritumab (10 μg/mL; 6 h) markedly inhibits the phosphorylation of HER3 and AKT, without affecting that of ERK, in DiFi-HRG4 cells[1].
Patritumab (10 μg/mL; 48 h) also induces the cleavage of PARP accompanied with both up-regulation of BIM and down-regulation of survivin expression[1].
Western Blot Analysis[1] | Cell Line: | DiFi-HRG cells | | Concentration: | 10 μg/mL | | Incubation Time: | 6 hours | | Result: | Inhibited the phosphorylation of HER3 and AKT as well as down-regulated survivin expression. |
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体内研究
(In Vivo) | Patritumab (1 mg/mouse; i.p.; twice a week for 4 weeks) combines with 1 mg Cetuximab and restores Cetuximab sensitivity in DiFi-HRG tumor xenografts model in mice[1].
Heregulin produced by colorectal cancer tumors harboring wild-type KRAS induces Cetuximab resistance, and that combination therapy with cetuximab and patritumab overcomes such resistance in vivo[1].
| Animal Model: | Female athymic nude mice (BALB/c; 5-6 weeks old) with DiFi-Mock1 or DiFi-HRG4 (s.c.)[1] | | Dosage: | 1 mg/body | | Administration: | Intraperitoneal injection; twice a week for 4 weeks | | Result: | Individual Patritumab treatment had little effect on the growth of tumors formed by either cell line.
Combination of Cetuximab and Patritumab induced substantial regression of DiFi-HRG4 xenografts. |
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| 运输条件 | Room temperature in continental US; may vary elsewhere. |
| 储存方式 | Please store the product under the recommended conditions in the Certificate of Analysis. |
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