Gap19 TFA, a peptide derived from nineamino acidsof the Cx43 cytoplasmic loop (CL), is a potent and selectiveconnexin 43 (Cx43) hemichannelblocker. Gap19 TFA inhibits hemichannels caused by preventing intramolecular interactions of the C-terminus (CT) with the CL. Gap19 TFA is not blocking GJ channels or Cx40/pannexin-1 hemichannels. Gap19 TFA has protective effects against myocardial^[1][2].

Cx43 Hemichannel^[1]

Gap19 TFA (250 μM; for 30 min ) decreases mitochondrial potassium uptake^[1].
Gap19 TFA (400 μM) inhibits unitary hemichannel currents in HeLa-Cx43 cells^[2].
Gap19 TFA (100 μM) inhibits hemichannel unitary currents in ventricular cardiomyocytes^[2].
Gap19 TFA (250 μM, 30 min) protects against myocardial ischemia/reperfusion injury in vitro and in vivo^[2].

Gap19 TFA (iv; 25 mg/kg; 10 min before ligation) significantly reduces the infarct size by approximately one-fifth^[2].

1275.46

Solid

C₅₇H₉₇F₃N₁₄O₁₅

KQIEIKKFK

Room temperature in continental US; may vary elsewhere.

Sealed storage, away from moisture

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

DMSO : 100 mg/mL(78.40 mM;Need ultrasonic)

H₂O : 100 mg/mL(78.40 mM;Need ultrasonic)

请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
储备液的保存方式和期限：-80℃, 6 months; -20℃, 1 month (sealed storage, away from moisture)。-80℃ 储存时，请在 6 个月内使用，-20℃ 储存时，请在 1 个月内使用。

请根据您的实验动物和给药方式选择适当的溶解方案。以下溶解方案都请先按照In Vitro方式配制澄清的储备液，再依次添加助溶剂：

——为保证实验结果的可靠性，澄清的储备液可以根据储存条件，适当保存；体内实验的工作液，建议您现用现配，当天使用； 以下溶剂前显示的百
分比是指该溶剂在您配制终溶液中的体积占比；如在配制过程中出现沉淀、析出现象，可以通过加热和/或超声的方式助溶

• 1.

  请依序添加每种溶剂： PBS

  Solubility: 100 mg/mL (78.40 mM); Clear solution; Need ultrasonic