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Cucurbitacin B
本产品不向个人销售,仅用作科学研究,不用于任何人体实验及非科研性质的动物实验。
Cucurbitacin B图片
CAS NO:6199-67-3
包装与价格:
包装价格(元)
10 mM * 1 mL in DMSO电议
5mg电议
10mg电议
25mg电议
50mg电议
100mg电议
200mg电议

产品名称
葫芦素 B
产品介绍
葫芦素B (Cucurbitacin B)是一具有抗癌活性的天然化合物。
生物活性

Cucurbitacin B belongs to a class of highly oxidized tetracyclic triterpenoids; could represscancercell progression. IC50 value: Target: anticancer natural compound in vitro: Cucurbitacin-B inhibited growth and modulated expression of cell-cycle regulators in SHSY5Y cells. At the molecular level, we found that Cucurbitacin-B inhibitedAKTsignaling activation through up-regulation ofPTEN[1]. CuB inducedapoptosisof A549 cells in a -concentration-dependent manner, as determined by fluorescence microscopy, flow cytometry and transmission electron microscopy. CuB dose-dependently inhibited lungcancercell proliferation, with cell cycle inhibition and cyclin B1 downregulation.Apoptosisinduced by CuB was shown to be associated with cytochrome c release, B-cell lymphoma 2 downregulation and signal transducer and activator of transcription 3 pathway inhibition [2]. CuB inhibited ITGA6 and ITGB4 (integrin α6 andintegrinβ4), which are overexpressed in breastcancer. Furthermore, CuB also induced the expression of major ITGB1and ITGB3, which are known to cause integrin-mediated cell death [3]. Cuc B treatment caused DNA double-strand breaks (DSBs) without affecting the signal transducer and activator of transcription 3 (STAT3), the potential molecular target for Cuc B. Cuc B triggers ATM-activated Chk1-Cdc25C-Cdk1, which could be reversed by bothATMsiRNA andChk1siRNA. Cuc B also triggers ATM-activated p53-14-3-3-σ pathways, which could be reversed byATMsiRNA [4]. in vivo: Efficacy of CuB was tested in vivo using two different orthotopic models of breastcancer. MDA-MB-231 and 4T-1 cells were injected orthotopically in the mammary fat pad of female athymic nude mice or BALB/c mice respectively. Our results showed that CuB administration inhibited MDA-MB-231 orthotopic tumors by 55%, and 4T-1 tumors by 40%. The 4T-1 cells represent stage IV breastcancerand form very aggressive tumors [3].

分子量

558.70

性状

Solid

Formula

C32H46O8

CAS 号

6199-67-3

中文名称

葫芦素 B;雪胆甲素;葫芦苦素 B

结构分类
  • Terpenoids
  • Triterpenes
来源
  • Plants
  • Cucurbitaceae
  • Trichosanthes cucumerinaL.
运输条件

Room temperature in continental US; may vary elsewhere.

储存方式
Powder-20°C3 years
4°C2 years
In solvent-80°C6 months
-20°C1 month
溶解性数据
In Vitro: 

DMSO : ≥ 100 mg/mL(178.99 mM)

H2O : 1 mg/mL(1.79 mM;Need ultrasonic)

*"≥" means soluble, but saturation unknown.

配制储备液
浓度溶剂体积质量1 mg5 mg10 mg
1 mM1.7899 mL8.9493 mL17.8987 mL
5 mM0.3580 mL1.7899 mL3.5797 mL
10 mM0.1790 mL0.8949 mL1.7899 mL
*

请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液;一旦配成溶液,请分装保存,避免反复冻融造成的产品失效
储备液的保存方式和期限:-80℃, 6 months; -20℃, 1 month。-80℃ 储存时,请在 6 个月内使用,-20℃ 储存时,请在 1 个月内使用。

In Vivo:

请根据您的实验动物和给药方式选择适当的溶解方案。以下溶解方案都请先按照In Vitro方式配制澄清的储备液,再依次添加助溶剂:

——为保证实验结果的可靠性,澄清的储备液可以根据储存条件,适当保存;体内实验的工作液,建议您现用现配,当天使用; 以下溶剂前显示的百
分比是指该溶剂在您配制终溶液中的体积占比;如在配制过程中出现沉淀、析出现象,可以通过加热和/或超声的方式助溶

  • 1.

    请依序添加每种溶剂: 10% DMSO    40%PEG300   5%Tween-80   45% saline

    Solubility: ≥ 2.5 mg/mL (4.47 mM); Clear solution

    此方案可获得 ≥ 2.5 mg/mL (4.47 mM,饱和度未知) 的澄清溶液。

    以 1 mL 工作液为例,取 100 μL 25.0 mg/mL 的澄清 DMSO 储备液加到 400 μL PEG300 中,混合均匀;向上述体系中加入50 μL Tween-80,混合均匀;然后继续加入 450 μL生理盐水定容至 1 mL。

    将 0.9 g 氯化钠,完全溶解于 100 mL ddH2O 中,得到澄清透明的生理盐水溶液
  • 2.

    请依序添加每种溶剂: 10% DMSO    90%corn oil

    Solubility: ≥ 2.5 mg/mL (4.47 mM); Clear solution

    此方案可获得 ≥ 2.5 mg/mL (4.47 mM,饱和度未知) 的澄清溶液,此方案不适用于实验周期在半个月以上的实验。

    以 1 mL 工作液为例,取 100 μL 25.0 mg/mL 的澄清 DMSO 储备液加到 900 μL玉米油中,混合均匀。

*以上所有助溶剂都可在本网站选购。