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AMPK-IN-3
本产品不向个人销售,仅用作科学研究,不用于任何人体实验及非科研性质的动物实验。
AMPK-IN-3图片
CAS NO:2417674-27-0
包装与价格:
包装价格(元)
5mg电议
10mg电议
25mg电议
50mg电议
100mg电议

产品介绍
AMPK-IN-3 (化合物 67) 是一种强效的、选择性的AMPK抑制剂,其对AMPK (α2)AMPK (α1)KDRIC50值分别为 60.7、107 和 3820 nM。AMPK-IN-3 对AMPK的抑制不影响细胞活力或导致 K562 细胞的明显细胞毒性。AMPK-IN-3 可用于癌症的研究。
生物活性

AMPK-IN-3 (compound 67) is a potent and selectiveAMPKinhibitor withIC50s of 60.7, 107 and 3820 nM forAMPK(α2),AMPK(α1)andKDR, respectively. AMPK-IN-3 inhibitsAMPKdoes not affect cell viability or cause significant cytotoxicity in K562 cells. AMPK-IN-3 can be used in study ofcancer[1].

IC50& Target[1]

AMPK (α2)

60.7 nM (IC50)

AMPK (α1)

107 nM (IC50)

KDR

3820 nM (IC50)

体外研究
(In Vitro)

AMPK-IN-3 (100 nM) shows inhibition values for AMPK(α2), FLT1, JAK1 JH2-pseudokinase and AMPK(α1) for 64%, 43%, 41% and 29%, respectively[1].
AMPK-IN-3 (0.195313, 0.78125, 3.125, 12.5, 50 μM; 2 h) decreases the level of p-ACC in K562 cells[1].
AMPK-IN-3 (1-100 μM; 24, 48, 72 h) shows potent inhibition of cellular AMPK activity but not affect cell viability[1].

Cell Viability Assay[1]

Cell Line:K562 cells
Concentration:0.195313, 0.78125, 3.125, 12.5, 50 μM
Incubation Time:2 h
Result:Decreased cellular levels of p-ACC(Ser79) in K562 cells.

Cell Viability Assay[1]

Cell Line:K562 cells
Concentration:1-100 μM
Incubation Time:24, 48, 72 h
Result:Showed no measurable impact on cell viability in K562 cells cultured under hypoxic conditions for 72 hours.
分子量

451.56

性状

Solid

Formula

C25H33N5O3

CAS 号

2417674-27-0

运输条件

Room temperature in continental US; may vary elsewhere.

储存方式
Powder-20°C3 years
4°C2 years
In solvent-80°C6 months
-20°C1 month
溶解性数据
In Vitro: 

DMSO : 115 mg/mL(254.67 mM;Need ultrasonic)

配制储备液
浓度溶剂体积质量1 mg5 mg10 mg
1 mM2.2145 mL11.0727 mL22.1455 mL
5 mM0.4429 mL2.2145 mL4.4291 mL
10 mM0.2215 mL1.1073 mL2.2145 mL
*

请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液;一旦配成溶液,请分装保存,避免反复冻融造成的产品失效
储备液的保存方式和期限:-80℃, 6 months; -20℃, 1 month。-80℃ 储存时,请在 6 个月内使用,-20℃ 储存时,请在 1 个月内使用。