Anandamide 是一种内源性大麻素。Anandamide 通过两个蛋白偶联的大麻素受体 (CB1和CB2) 调节神经元和免疫功能。Anandamide 可激活多种受体，如PPARS、TRPV1和GPR18/GPR55。Anandamide 还具有抗真菌 (anti-fungal) 活性和抗炎 (anti-inflammatory) 活性。Anandamide 可用于阿尔茨海默病 (AD) 和溃疡性结肠炎的研究。
| 生物活性 | Anandamide is an endocannabinoid. Anandamide modulates both neuronal and immune functions through two protein-coupled cannabinoid receptors (CB1andCB2). Anandamide can activate numerous other receptors likePPARS,TRPV1, andGPR18/GPR55. Anandamide also has potentialanti-fungalandanti-inflammatoryactivities. Anandamide can be used for the research of Alzheimer’s disease (AD) and ulcerative colitis[1][2][3][4][5]. |
| IC50& Target[1] | CB1 | CB2 | PPAR | TRPV1 | Microbial Metabolite | Human Endogenous Metabolite |
|
体外研究
(In Vitro) | Anandamide (0-250 μg/ml, 1 h) inhibitsC. albicanshyphal growth and prevents hyphal adherence to epithelial cells[4].
Anandamide (0-250 μg/ml, 2 h) alters the expression of genes involved in adhesion and hyphal morphogenesis[4].
Anandamide reduces tau phosphorylation through the inhibition of the activity of protein kinases[3].
RT-PCR[4] | Cell Line: | HeLa cervical epithelial cells | | Concentration: | 0, 10, 50, 125, 250 μg/ml | | Incubation Time: | 2 h | | Result: | Repressed the expression of the HWP1 and ALS3 adhesins involved in Candida adhesion to epithelial cells and the HGC1, RAS1, EFG1 and ZAP1 regulators of hyphal morphogenesis and cell adherence. Increased the expression of NRG1, a transcriptional repressor of filamentous growth. |
|
体内研究
(In Vivo) | Anandamide (10 mg/kg, IP, once) considerably lowers the increase of glycemia in response to glucose ingestion compared with control, and this is associated with an improvement of glucose tolerance[2].
Anandamide (100 ng, ICV bilateral injection, single) partially prevents streptozotocin (STZ)-induced cognitive impairments, changes in synaptic markers and ventricle enlargement[3].
Anandamide exerts anti-inflammatory activities, attenuating the development of inflammation in a mouse model of ulcerative colitis[4].
Anandamide alleviates lipopolysaccharide (LPS)-induced neuroinflammation in rat primary microglial cultures[1].
| Animal Model: | Male Wistar rats (3-4 months, 350-400 g, STZ-induced AD-like sporadic dementia model)[3] | | Dosage: | 100 ng | | Administration: | ICV bilateral injection, single | | Result: | Prevented recognition and non-associative emotional memory impairments induced by STZ, but did not influence tone fear conditioning. Decreased BAG2 (Bcl2-associated athanogene) levels, and prevented STZ-induced ventricle enlargement. |
|
| 分子量 | |
| 性状 | |
| Formula | |
| CAS 号 | |
| 中文名称 | |
| 结构分类 | - Ketones, Aldehydes, Acids
|
| 运输条件 | Room temperature in continental US; may vary elsewhere. |
| 储存方式 | -20°C, stored under nitrogen *In solvent : -80°C, 6 months; -20°C, 1 month (stored under nitrogen) |
| 溶解性数据 | In Vitro: DMSO : ≥ 100 mg/mL(287.74 mM) *"≥" means soluble, but saturation unknown. 配制储备液 | 1 mM | 2.8774 mL | 14.3872 mL | 28.7745 mL | | 5 mM | 0.5755 mL | 2.8774 mL | 5.7549 mL | | 10 mM | 0.2877 mL | 1.4387 mL | 2.8774 mL |
*请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
储备液的保存方式和期限：-80℃, 6 months; -20℃, 1 month (stored under nitrogen)。-80℃ 储存时，请在 6 个月内使用，-20℃ 储存时，请在 1 个月内使用。 In Vivo: 请根据您的实验动物和给药方式选择适当的溶解方案。以下溶解方案都请先按照In Vitro方式配制澄清的储备液，再依次添加助溶剂： ——为保证实验结果的可靠性，澄清的储备液可以根据储存条件，适当保存；体内实验的工作液，建议您现用现配，当天使用；
以下溶剂前显示的百
分比是指该溶剂在您配制终溶液中的体积占比；如在配制过程中出现沉淀、析出现象，可以通过加热和/或超声的方式助溶 1. 请依序添加每种溶剂： 10% DMSO 40%PEG300 5%Tween-80 45% saline Solubility: ≥ 2.08 mg/mL (5.99 mM); Clear solution
此方案可获得 ≥ 2.08 mg/mL (5.99 mM，饱和度未知) 的澄清溶液。 以 1 mL 工作液为例，取 100 μL 20.8 mg/mL 的澄清 DMSO 储备液加到 400 μL PEG300 中，混合均匀；向上述体系中加入50 μL Tween-80，混合均匀；然后继续加入 450 μL生理盐水定容至 1 mL。 2. 请依序添加每种溶剂： 10% DMSO 90% (20%SBE-β-CDin saline) Solubility: ≥ 2.08 mg/mL (5.99 mM); Clear solution
此方案可获得 ≥ 2.08 mg/mL (5.99 mM，饱和度未知) 的澄清溶液。 以 1 mL 工作液为例，取 100 μL 20.8 mg/mL 的澄清 DMSO 储备液加到 900 μL20% 的 SBE-β-CD 生理盐水水溶液中，混合均匀。 3. 请依序添加每种溶剂： 10% DMSO 90%corn oil Solubility: ≥ 2.08 mg/mL (5.99 mM); Clear solution
此方案可获得 ≥ 2.08 mg/mL (5.99 mM，饱和度未知) 的澄清溶液，此方案不适用于实验周期在半个月以上的实验。 以 1 mL 工作液为例，取 100 μL 20.8 mg/mL 的澄清 DMSO 储备液加到 900 μL玉米油中，混合均匀。 *以上所有助溶剂都可在本网站选购。
|
m.cnreagent.com