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Tocilizumab
本产品不向个人销售,仅用作科学研究,不用于任何人体实验及非科研性质的动物实验。
CAS NO:375823-41-9
包装与价格:
包装价格(元)
1mg电议
5mg电议
10mg电议
50mg电议

产品名称
托珠单抗
Anti-Human IL6R, Humanized Antibody
产品介绍
Tocilizumab (Anti-Human IL6R, Humanized Antibody) 是一种抗人白细胞介素-6 受体 (IL-6R) 的中和抗体,可阻止 IL-6 与 IL-6R 的结合,从而抑制经典和反式信号。Tocilizumab 可用于类风湿性关节炎的相关研究。Tocilizumab 在重症 COVID-19 病毒方面的研究效果显著。
生物活性

Tocilizumab (Anti-Human IL6R, Humanized Antibody) is an anti-human interleukin-6 receptor (IL-6R) neutralizing antibody, prevents binding ofIL-6to theIL-6R, thereby inhibiting both classic and trans-signaling. Tocilizumab (Anti-Human IL6R, Humanized Antibody) can be used for the treatment of rheumatoid arthritis[1]. Tocilizumab is remarkablely effective for the study of severe COVID-19 (coronavirus disease)[4].

IC50& Target[1][2]

IL-6

 

体外研究
(In Vitro)

Tocilizumab (Anti-Human IL6R, Humanized Antibody) (1-100 ng/mL; 24 hours, 48 hours) inhibits the cell proliferation (growth rate) in a dose-dependent manner, growth rate by HACM is reduced almost completely by 100 ng/mL tocilizumab[2].
Tocilizumab (Anti-Human IL6R, Humanized Antibody) (0.001-10,000 μg/ml; 2 days) inhibits Ba/F3-gp130-IL-6R cell proliferation stimulated with hIL-6 with an IC50of 13.5 ng/ml[1].
Tocilizumab (Anti-Human IL6R, Humanized Antibody) (1 μM; 6 hours) reduces the phosphorylation of STAT3 in vitro consistent with in vivo SAS cells[3].
Tocilizumab (Anti-Human IL6R, Humanized Antibody) (1-5 μM; 24 hours) significantly reduces VEGF mRNA expression in SAS cells with serum-free medium[3].

Cell Proliferation Assay[2]

Cell Line:Human-derived HARA-B cells
Concentration:1 ng/mL, 10 ng/mL, and 100 ng/mL
Incubation Time:24 hours, 48 hours
Result:Inhibited the IL-6R expressed on human-derived HARA-B cells.

Cell Cytotoxicity Assay[1]

Cell Line:Ba/F3-gp130-IL-6R cells
Concentration:0.001–10000 μg/ml
Incubation Time:2 days
Result:Inhibited Ba/F3-gp130-IL-6R cell proliferation.

Western Blot Analysis[3]

Cell Line:SAS cells
Concentration:1 μM
Incubation Time:6 hours
Result:Reduced the phosphorylation of STAT3.

RT-PCR[3]

Cell Line:SAS cells
Concentration:1-5 μM
Incubation Time:24 hours
Result:Reduced VEGF mRNA expression.
体内研究
(In Vivo)

Tocilizumab (Anti-Human IL6R, Humanized Antibody) (intravenous injection; 1.0 mg/100 μL; twice a week, three weeks) decreases the volume of each metastatic focus in tocilizumab-injected mouse brains[2].
Tocilizumab (Anti-Human IL6R, Humanized Antibody) (intraperitoneal injection;100 μg/mL, every 48 hours; 20days) suppresses OSCC progression and suppresses Tumor Angiogenesis[3].

Animal Model:A brain metastasis model of lung tumor cells by inoculating HARA-B cells in nude mice[2]
Dosage:1.0 mg/100 μL
Administration:Intravenous injection; 1.0 mg/100 μL; twice a week, three weeks
Result:Led the volume of each metastatic focus significantly smaller.
Animal Model:SCID mice with SAS cells[3]
Dosage:100 μg/mL
Administration:Intraperitoneal injection;100 μg/mL; every 48 hours; 20days
Result:Suppressed the growth of advanced tumors.
Clinical Trial
性状

Liquid

CAS 号

375823-41-9

中文名称

托珠单抗

运输条件

Room temperature in continental US; may vary elsewhere.

储存方式

Please store the product under the recommended conditions in the Certificate of Analysis.