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Colivelin TFA
本产品不向个人销售,仅用作科学研究,不用于任何人体实验及非科研性质的动物实验。
包装与价格:
包装价格(元)
500 μg电议
1mg电议
5mg电议
10mg电议
50mg电议
100mg电议

产品介绍
Colivelin TFA 是一种具有大脑通透性的神经保护肽 (neuroprotective peptide),是STAT3的有效激活剂。Coliveli 通过激活 STAT3 在体外抑制神经元死亡。Colivelin TFA 对神经毒性、Aβ 沉积,神经元细胞凋亡,在神经退行性疾病显示出长期有利的影响。Colivelin TFA 可用于阿尔茨海默病和缺血性脑损伤的相关研究。
生物活性

Colivelin TFA is a brain penetrantneuroprotective peptideand a potent activator ofSTAT3, suppresses neuronal death by activating STAT3 in vitro[1]. Colivelin TFA exhibits long-term beneficial effects against neurotoxicity, Aβ deposition, neuronalapoptosis, and synaptic plasticity deficits in neurodegenerative disease[2]. Colivelin TFA has the potential for the treatment of alzheimer's disease and ischemic brain injury[1].

IC50& Target[1]

STAT3

 

Amyloid-β

 

体外研究
(In Vitro)

Colivelin completely suppresses death induced by overexpressed FAD-causative genes and Aβ1-43 at a concentration of 100 fm, and keep its neuroprotective action at or above the levels of 1 nm[1].
Colivelin-induced neuroprotection occurs via two neuroprotective pathways: one mediated by Ca2+/calmodulin-dependent protein kinase IV, triggered by ADNF, and one mediated by signal transducer and activator of transcription 3, triggered by HN[1].
Colivelin reverses caspase3, Bax and Bcl-2 expressions in HT22 cells medaited by rmMFG-E8 in the co-cultured cells under OGD condition[4].
Colivelin (50 μg/mL, 4 hours) significantly increases the p-STAT3 protein levels in BV-2 cells[4].

Western Blot Analysis[4]

Cell Line:BV-2 cells.
Concentration:50 μg/mL.
Incubation Time:4 hours.
Result:Increased p-STAT3 levels.

Cell Viability Assay[5]

Cell Line:KYSE70 and TE8 cells.
Concentration:0.5 μM.
Incubation Time:1 hour (followed by CYT-Rx20 treatment)
Result:Significantly suppressed the viability in KYSE70 and TE8 cells.
体内研究
(In Vivo)

Colivelin(intracerebroventricular administration; 10 pmol/3 μl; 3 weeks) suppresses impairment in spatial working memory induced by repetitive intracerebroventricular injection of Aβ25-35 or Aβ1-42, in addition, it antagonizes neuronal loss in the CA1 region of hippocampus induced by hippocampal injection of Aβ1-42[1].
Colivelin (intraperitoneal administration; 1.4, 7, or 35 nM/0.21mL; on the Y-maze testday) suppresses memory impairment caused by 3-quinuclidinyl benzilateand restricts functional memory deficit[1].
Colivelin (intraperitoneal injection; 1 mg/kg; 14 days) results in improved motor and cognitive function with time by performance of mNSS, rotarod, and corner turning test.It also reduces lesion volume and improves neurological deficits after MCAO[1].

Animal Model:CD-1 mice[1]
Dosage:10 pmol/3 μl
Administration:Intracerebroventricular administration
Result:Completely suppressed Aβ 25-35-mediated impairment in spatial working memory and increased the number of immunoreactive neurons.
Animal Model:C57 mice[1]
Dosage:1.4, 7, or 35 nM/0.21mL
Administration:Intraperitoneal administration
Result:Protected against cholinotoxin-induced amnesia in mice.
Animal Model:Male C57BL/6 mice[3]
Dosage:1 mg/kg
Administration:Intraperitoneal administration
Result:Protected against ischemic brain injury, and improves neurological outcomes.
分子量

2759.12

性状

Solid

Formula

C119H206N32O35.C2HF3O2

Sequence Shortening

SALLRSIPAPAGASRLLLLTGEIDLP

运输条件

Room temperature in continental US; may vary elsewhere.

储存方式

Sealed storage, away from moisture

Powder-80°C2 years
-20°C1 year

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

溶解性数据
In Vitro: 

H2O : 50 mg/mL(18.12 mM;Need ultrasonic)

配制储备液
浓度溶剂体积质量1 mg5 mg10 mg
1 mM0.3624 mL1.8122 mL3.6243 mL
5 mM0.0725 mL0.3624 mL0.7249 mL
10 mM0.0362 mL0.1812 mL0.3624 mL
*

请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液;一旦配成溶液,请分装保存,避免反复冻融造成的产品失效
储备液的保存方式和期限:-80℃, 6 months; -20℃, 1 month (sealed storage, away from moisture)。-80℃ 储存时,请在 6 个月内使用,-20℃ 储存时,请在 1 个月内使用。

In Vivo:

请根据您的实验动物和给药方式选择适当的溶解方案。以下溶解方案都请先按照In Vitro方式配制澄清的储备液,再依次添加助溶剂:

——为保证实验结果的可靠性,澄清的储备液可以根据储存条件,适当保存;体内实验的工作液,建议您现用现配,当天使用; 以下溶剂前显示的百
分比是指该溶剂在您配制终溶液中的体积占比;如在配制过程中出现沉淀、析出现象,可以通过加热和/或超声的方式助溶

  • 1.

    请依序添加每种溶剂: Water with 5% sefsol and 20% isopropanol

    Solubility: 6.25 mg/mL (2.27 mM); Suspended solution; Need ultrasonic

*以上所有助溶剂都可在本网站选购。