MMI-0100

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MMI-0100

本产品不向个人销售，仅用作科学研究，不用于任何人体实验及非科研性质的动物实验。

CAS NO:

1039342-24-9

包装与价格：

包装	价格(元)
5mg	电议
10mg	电议
25mg	电议
50mg	电议
100mg	电议

产品介绍

MMI-0100 是一种细胞渗透性肽抑制剂，抑制丝裂原活化蛋白激酶活化蛋白激酶 II (MK2)。MMI-0100 在体内外减少内膜增生。MMI-0100 抑制 IL-6 表达而不影响 IL-8 表达。MMI-0100 抑制纤维化过程，例如静脉移植疾病。

生物活性

MMI-0100 is a cell-permeant peptide inhibitor of mitogen activated protein kinase activated protein kinase II (MK2). MMI-0100 reduces intimal hyperplasia ex vivo and in vivo. MMI-0100 suppressesIL-6expression without effect onIL-8expression. MMI-0100 suppresses fibrotic processes such as vein graft disease^[1].

体外研究
(In Vitro)

Naphthofluorescein (compound 19) (0-10 μM; 24 hours) suppresses the HIF-1 reporter activity in a concentration-dependent manner.^[1].
MMI-0100 (0.25 and 0.5 mM; 24 hours) slightly increases cell proliferation in both cell types compared to control cells treated with 20 ng/ml TNF-α alone^[1].
MMI-0100 (1 mM) treatment also increases both EC (11%) and SMC (7%) proliferation as compared to control, this response is not as robust as that induced by treatment with 0.5 mM MMI-0100^[1].
MMI-0100 does not induce EC apoptosis at any dose^[1].

Cell Viability Assay^[1]

Cell Line:	human endothelial cell (EC) and smooth muscle cell (SMC)
Concentration:	0.25, 0.5, and 1 mM
Incubation Time:	24 hours
Result:	Slightly increased cell proliferation in both cell types compared to control cells.

体内研究
(In Vivo)

MMI-0100 (100 μM; 28 days) inhibits intimal hyperplasia in a mouse vein graft model^[1].

Animal Model:	12-week-old C57Bl/6 wild type mice (intimal hyperplasia)^[1]
Dosage:	100 μM
Administration:	vein graft, 28 days
Result:	Diminished wall thickness at all postoperative time points in vein grafts treated with MMI-0100, with a ratio of 2.6-fold thicker at 4 weeks, compared to 4.7-fold thicker at 4 weeks in control grafts.

Clinical Trial

分子量

2283.64

性状

Solid

Formula

C₉₈H₁₇₁N₃₇O₂₆

CAS 号

1039342-24-9

Sequence Shortening

YARAAARQARAKALARQLGVAA

运输条件

Room temperature in continental US; may vary elsewhere.

储存方式

Sealed storage, away from moisture and light, under nitrogen

Powder	-80°C	2 years
	-20°C	1 year

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light, under nitrogen)

溶解性数据

In Vitro:

H₂O : 100 mg/mL(43.79 mM;Need ultrasonic)

配制储备液

浓度溶剂体积质量

1 mg

5 mg

10 mg

1 mM	0.4379 mL	2.1895 mL	4.3790 mL
5 mM	0.0876 mL	0.4379 mL	0.8758 mL
10 mM	0.0438 mL	0.2189 mL	0.4379 mL

请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
储备液的保存方式和期限：-80℃, 6 months; -20℃, 1 month (sealed storage, away from moisture and light, under nitrogen)。-80℃ 储存时，请在 6 个月内使用，-20℃ 储存时，请在 1 个月内使用。

In Vivo:

请根据您的实验动物和给药方式选择适当的溶解方案。以下溶解方案都请先按照In Vitro方式配制澄清的储备液，再依次添加助溶剂：

——为保证实验结果的可靠性，澄清的储备液可以根据储存条件，适当保存；体内实验的工作液，建议您现用现配，当天使用；以下溶剂前显示的百
分比是指该溶剂在您配制终溶液中的体积占比；如在配制过程中出现沉淀、析出现象，可以通过加热和/或超声的方式助溶

1.
请依序添加每种溶剂： PBS
Solubility: 14.29 mg/mL (6.26 mM); Clear solution; Need ultrasonic

*以上所有助溶剂都可在本网站选购。