SN 6 is a selectiveNa⁺/Ca²⁺exchanger (NCX)inhibitor, and inhibits⁴⁵Ca²⁺uptake by NCX1, NCX2, and NCX3, withIC₅₀s of 2.9, 16, and 8.6 μM, respectively.

IC50：2.9 μM (NCX1), 16 μM (NCX2), 8.6 μM (NCX3)^[1]

SN 6 is a selective Na⁺/Ca²⁺exchanger inhibitor, which inhibits the initial rate of⁴⁵Ca²⁺uptake into NCX1, NCX2, and NCX3 transfectants with IC₅₀values of 2.9 ± 0.12, 16 ± 1.1, and 8.6 ± 0.27 μM. SN 6 (up to 30 μM) also less potently inhibits muscarinic acetylcholine receptor, with a higher IC₅₀of 18 μM. SN 6 (0.3-30 μM) completely inhibits the initial rate of Na⁺_i-dependent⁴⁵Ca²⁺uptake into Na⁺-loaded sarcolemmal vesicles in a dose dependent manner (IC₅₀, 5.3 ± 0.37 μM). SN 6 (0.3-10 μM) dose-dependently protects against the hypoxia/reoxygenation-induced LDH release in parental LLC-PK1 cells and NCX1 transfectants but not in K229Q transfectants^[1]. SN 6 (1-30 μM) suppresses the bidirectional outward and inward I_NCXin a concentration-dependent manner, with IC₅₀values of 2.3 μM and 1.9 μM, respectively. SN 6 also inhibits bidirectional current (I_NCX) in a [Na⁺]i concentration-dependent manner, with IC₅₀values of 3.4 μM, 2.3 μM, and 1.1 μM at 10 mM, 20 mM, and 30 mM [Na⁺]i, respectively^[2]. SN 6 inhibits hypoxia/reoxygenation-induced LDH release with an IC₅₀value of 0.63 ± 0.15 μM in NCX1 transfectants^[3].

402.46

Solid

C₂₀H₂₂N₂O₅S

415697-08-4

Room temperature in continental US; may vary elsewhere.

DMSO : 62.5 mg/mL(155.29 mM;Need ultrasonic)

请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
储备液的保存方式和期限：-80℃, 6 months; -20℃, 1 month。-80℃ 储存时，请在 6 个月内使用，-20℃ 储存时，请在 1 个月内使用。

请根据您的实验动物和给药方式选择适当的溶解方案。以下溶解方案都请先按照In Vitro方式配制澄清的储备液，再依次添加助溶剂：

——为保证实验结果的可靠性，澄清的储备液可以根据储存条件，适当保存；体内实验的工作液，建议您现用现配，当天使用； 以下溶剂前显示的百
分比是指该溶剂在您配制终溶液中的体积占比；如在配制过程中出现沉淀、析出现象，可以通过加热和/或超声的方式助溶

• 1.

  请依序添加每种溶剂： 10% DMSO    40%PEG300   5%Tween-80   45% saline

  Solubility: 2.08 mg/mL (5.17 mM); Suspended solution; Need ultrasonic and warming

  此方案可获得 2.08 mg/mL (5.17 mM) 的均匀悬浊液，悬浊液可用于口服和腹腔注射。

  以 1 mL 工作液为例，取 100 μL 20.8 mg/mL 的澄清 DMSO 储备液加到 400 μL PEG300 中，混合均匀；向上述体系中加入50 μL Tween-80，混合均匀；然后继续加入 450 μL生理盐水定容至 1 mL。

  将 0.9 g 氯化钠，完全溶解于 100 mL ddH2O 中，得到澄清透明的生理盐水溶液
• 2.

  请依序添加每种溶剂： 10% DMSO    90%corn oil

  Solubility: 2.08 mg/mL (5.17 mM); Clear solution; Need warming

  此方案可获得 2.08 mg/mL (5.17 mM) 的澄清溶液，此方案不适用于实验周期在半个月以上的实验。

  以 1 mL 工作液为例，取 100 μL 20.8 mg/mL 的澄清 DMSO 储备液加到 900 μL玉米油中，混合均匀。