Scoulerine ((-)-Scoulerine) 是一种有效的抗有丝分裂 (antimitotic) 化合物。Scoulerine 也是BACE1(淀粉样前体蛋白裂解酶 1) 的抑制剂。Scoulerine 抑制细胞增殖，阻止细胞周期，并诱导癌细胞凋亡。
| 生物活性 | Scoulerine ((-)-Scoulerine), an isoquinoline alkaloid, is a potentantimitoticcompound. Scoulerine is also an inhibitor ofBACE1(ss-site amyloid precursor protein cleaving enzyme 1). Scoulerine inhibits proliferation, arrests cell cycle, and inducesapoptosisincancercells[1]. |
体外研究
(In Vitro) | Scoulerine ((-)-Scoulerine) inhibits mini-panel of human leukemic cells, MOLT-4 (WT), Jurkat (TP53 mutated), Raji (TP53 mutated), HL-60 (TP53 null), U-937 (TP53 mutated), and HEL 92.1.7 (wild-type), with IC50s ranging from 2.7 μM to 6.5 μM[1].
Scoulerine (2.5-20 μM; 24 hours) decreases proliferation of Jurkat and MOLT-4 cells[1].
Scoulerine (2.5-20 μM; 24 hours) induces MOLT-4 and Jurkat cells apoptosis[1].
Scoulerine induces G2 or M cell cycle arrest[1].
Scoulerine (2.5-5 μM; 24 hours) shows an upregulation of p53 protein in p53 wild-type MOLT-4 cells[1].
Scoulerine (2.5-5 μM; 24-48 hours) activates caspase-3/7, -8 and -9 in a dose-dependent manner[1].
Scoulerine (5-10 μM; 24 hours) disrupts microtubule structure of A549 lung carcinoma cells[1]
Cell Proliferation Assay[1] | Cell Line: | Jurkat and MOLT-4 cells | | Concentration: | 2.5, 5, 10, 15 and 20 μM | | Incubation Time: | 24 hours | | Result: | Significantly reduced the viability and proliferation of Jurkat and MOLT-4 cells in a dose dependent manner. |
Apoptosis Analysis[1] | Cell Line: | MOLT-4 and Jurkat cells | | Concentration: | 2.5, 5, 10, 15 and 20 μM | | Incubation Time: | 24 hours | | Result: | Induced MOLT-4 and Jurkat cells apoptosis. |
Cell Cycle Analysis[1] | Cell Line: | Jurkat and MOLT-4 leukemic cells | | Concentration: | 2.5-20 μM | | Incubation Time: | 16 hours | | Result: | Induced cell cycle arrest at the G2/M transition. |
Western Blot Analysis[1] | Cell Line: | MOLT-4 cells | | Concentration: | 2.5, 5 μM | | Incubation Time: | 24 hours | | Result: | Showed an upregulation of p53 protein in p53 wild-type MOLT-4 cells. |
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| 结构分类 | - Alkaloids
- Isoquinoline Alkaloids
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| 运输条件 | Room temperature in continental US; may vary elsewhere. |
| 储存方式 | -20°C, sealed storage, away from moisture and light *In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light) |
| 溶解性数据 | In Vitro: DMSO : 100 mg/mL(305.46 mM;Need ultrasonic) 配制储备液 | 1 mM | 3.0546 mL | 15.2732 mL | 30.5465 mL | | 5 mM | 0.6109 mL | 3.0546 mL | 6.1093 mL | | 10 mM | 0.3055 mL | 1.5273 mL | 3.0546 mL |
*请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
储备液的保存方式和期限：-80℃, 6 months; -20℃, 1 month (sealed storage, away from moisture and light)。-80℃ 储存时，请在 6 个月内使用，-20℃ 储存时，请在 1 个月内使用。 |
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